CSK Antibody
Tyrosine-protein kinase CSK, C-Src kinase, Protein-tyrosine kinase CYL, CSK, C-Src Tyrosine Kinase.
Description
Product Specs
C-Src Tyrosine Kinase, also called CSK, is a key regulator of cellular processes such as growth, movement, specialization, and immune response. It achieves this by phosphorylating tyrosine residues within the C-terminal tails of Src-family kinases. The CSK gene encodes a protein that acts as a suppressor of signaling pathways initiated by various cell surface receptors. Important pathways associated with CSK include the PI-3K cascade and signaling pathways activated by fibroblast growth factor receptors (FGFR).
The product appears as a colorless solution that has been sterilized through filtration.
This solution is provided at a concentration of 1mg/ml and contains the following components: phosphate-buffered saline (PBS) at a pH of 7.4, glycerol at a concentration of 10%, and sodium azide at a concentration of 0.02%.
For short-term storage of up to one month, the product should be kept at a temperature of 4°C. For prolonged storage, it is recommended to store the product at -20°C. Repeated freezing and thawing of the product should be avoided.
The product has a shelf life of 12 months when stored at -20°C and a shelf life of 1 month when stored at 4°C.
The CSK antibody has undergone rigorous testing using methods such as ELISA, Western blot analysis, and immunocytochemistry/immunofluorescence (ICC/IF) to ensure its specificity and reactivity. However, it's important to note that optimal working dilutions may vary depending on the specific application, and therefore, users are advised to perform their own titrations to determine the optimal conditions for their experiments.
Tyrosine-protein kinase CSK, C-Src kinase, Protein-tyrosine kinase CYL, CSK, C-Src Tyrosine Kinase.
CSK antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT7C6AT.
Anti-human CSK mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CSK amino acids 1-450 purified from E. coli.
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
C-Src tyrosine kinase, also known as proto-oncogene c-Src, is a non-receptor tyrosine kinase protein encoded by the SRC gene in humans. It belongs to the Src family kinases, which includes several members such as Yes, Fyn, Fgr, Yrk, Lyn, Blk, Hck, and Lck . The c-Src protein plays a crucial role in various cellular processes, including embryonic development, cell growth, and differentiation .
The discovery of c-Src dates back to 1979 when American scientists J. Michael Bishop and Harold E. Varmus identified that normal chickens possess a gene structurally related to the v-Src gene of the Rous sarcoma virus . This discovery led to a paradigm shift in cancer research, demonstrating that cancer could be caused by a gene normally present in the cell rather than a foreign substance. For their groundbreaking work, Bishop and Varmus were awarded the Nobel Prize in Physiology or Medicine in 1989 .
C-Src is composed of several domains, including an SH2 domain, an SH3 domain, and a tyrosine kinase domain . These domains are essential for its function in phosphorylating specific tyrosine residues in other proteins, thereby regulating various signaling pathways. The activity of c-Src is tightly regulated, and its deregulation has been implicated in cancer progression .
Elevated levels of c-Src activity have been linked to the malignant progression of various cancers, including colon cancer . Mutations in the c-Src gene can lead to uncontrolled cell growth and division, contributing to tumor development. The role of c-Src in cancer has made it a target for therapeutic interventions, with several inhibitors being developed to target its activity .
Mouse anti-human c-Src tyrosine kinase antibodies are commonly used in research to study the expression and function of c-Src in human cells. These antibodies are generated by immunizing mice with human c-Src protein, leading to the production of specific antibodies that can bind to and detect c-Src in various assays. These antibodies are valuable tools for investigating the role of c-Src in cellular processes and disease states.
