CSF2RB Human
Description
CSF2RB Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 435 amino acids (17-443 a.a) and having a molecular mass of 49.7kDa.
CSF2RB is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that plays a crucial role in the immune system by stimulating the production and function of white blood cells, particularly granulocytes and macrophages . GM-CSF is produced by various cell types, including T cells, B cells, macrophages, monocytes, mast cells, vascular endothelial cells, and fibroblasts .
The GM-CSF receptor is a heterodimer composed of two subunits: the alpha (α) subunit and the beta (β) subunit . The alpha subunit (GMRα) binds GM-CSF with low affinity, while the beta subunit (βc) is responsible for signal transduction and is shared with the receptors for interleukin-3 (IL-3) and interleukin-5 (IL-5) . The βc subunit is expressed at lower levels than GMRα on the surface of leukocytes .
Upon binding of GM-CSF to its receptor, the Janus-kinase-2 (JAK-2) is recruited to the cytoplasmic domain of the β subunit, leading to the activation of JAK-2 and subsequent phosphorylation of STAT-5 . This signaling pathway induces the migration of STAT-5 dimers to the nucleus, promoting the transcription of various genes involved in cell differentiation . The GM-CSF receptor is primarily found in myeloid cells and some non-hematopoietic cells, but it is not expressed by lymphoid cells such as T cells .
Recombinant human GM-CSF receptor beta (βc) is produced using recombinant DNA technology, which involves inserting the gene encoding the β subunit into a suitable expression system, such as bacteria or mammalian cells . The recombinant protein is then purified and used for various research and therapeutic applications .
Recombinant human GM-CSF receptor beta is used in research to study the signaling pathways and functions of GM-CSF in various cell types . It is also used in the development of therapeutic strategies targeting GM-CSF signaling in autoimmune diseases and cancer . For example, sargramostim, a recombinant human GM-CSF, has been approved by the US FDA to accelerate bone marrow recovery in patients with bone marrow failure .
