CRYZ Human

Crystallin Zeta Human Recombinant

Cat. No.

BT21028

Source

Escherichia Coli.

Synonyms

Quinone oxidoreductase, NADPH:quinone reductase, Zeta-crystallin, CRYZ, Quinone oxidoreductase isoform a, Crystallin, zeta (quinone reductase), Crystallin Zeta.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

CRYZ Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 352 amino acids (1-329 a.a.) and having a molecular mass of 37.6kDa.
CRYZ is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Crystallin Zeta, also called CRYZ, is a protein involved in several biological processes. It binds to NADP and plays a role in one-electron transfer reactions. CRYZ is involved in detoxifying foreign substances (xenobiotics) and interacts with specific regions rich in adenine and uracil nucleotides (AU-rich elements or AREs) located in the 3' untranslated region (3'-UTR) of messenger RNA (mRNA) molecules. Specifically, CRYZ enhances the production of BCL2 protein by interacting with its mRNA.

Description

Recombinant human CRYZ protein was produced in E. coli bacteria. It is a single polypeptide chain that lacks glycosylation modifications and consists of 352 amino acids (specifically, amino acids 1 to 329). This protein has a molecular weight of 37.6 kDa. The recombinant CRYZ protein is engineered with a 23 amino acid His-tag attached to its N-terminus to facilitate purification using proprietary chromatographic methods.

Physical Appearance

The product is a clear, sterile-filtered solution with no color.

Formulation

The CRYZ protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution contains the following components: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.

Stability

For short-term storage (up to 2-4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advisable. Repeated freezing and thawing of the product should be avoided.

Purity

The purity of the CRYZ protein is greater than 95%, as determined by SDS-PAGE analysis.

Synonyms

Quinone oxidoreductase, NADPH:quinone reductase, Zeta-crystallin, CRYZ, Quinone oxidoreductase isoform a, Crystallin, zeta (quinone reductase), Crystallin Zeta.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMATGQKL MRAVRVFEFG GPEVLKLRSD IAVPIPKDHQ VLIKVHACGV NPVETYIRSG TYSRKPLLPY TPGSDVAGVI EAVGDNASAF KKGDRVFTSS TISGGYAEYA LAADHTVYKL PEKLDFKQGA AIGIPYFTAY RALIHSACVK AGESVLVHGA SGGVGLAACQ IARAYGLKIL GTAGTEEGQK IVLQNGAHEV FNHREVNYID KIKKYVGEKG IDIIIEMLAN VNLSKDLSLL SHGGRVIVVG SRGTIEINPR DTMAKESSII GVTLFSSTKE EFQQYAAALQ AGMEIGWLKP VIGSQYPLEK VAEAHENIIH GSGATGKMIL LL.

Product Science Overview

Introduction

Crystallins are a family of proteins primarily known for their role in maintaining the transparency and refractive index of the vertebrate eye lens. They are categorized into two main classes: taxon-specific (or enzyme) crystallins and ubiquitous crystallins. Crystallin Zeta (CRYZ), also known as Zeta-crystallin, belongs to the taxon-specific class and is encoded by the CRYZ gene.

Structure and Function

Crystallin Zeta is a protein-coding gene that produces a protein with NADPH-dependent quinone reductase activity. This activity is distinct from other known quinone reductases and does not include alcohol dehydrogenase activity, despite being a member of the zinc-containing alcohol dehydrogenase family. The protein binds NADP and acts through a one-electron transfer process, with orthoquinones such as 1,2-naphthoquinone or 9,10-phenanthrenequinone being the best substrates in vitro.

Biological Role

Crystallin Zeta has two main functions:

mRNA Binding and Stabilization: It binds to mRNA species, particularly those with AU-rich elements (ARE) in their 3’-UTR, enhancing the stability of these mRNAs. This includes mRNAs coding for antiapoptotic proteins like BCL2.
Enzymatic Activity: It acts as an NADPH:quinone oxidoreductase, potentially playing a role in the detoxification of xenobiotics.

Expression and Localization

In humans, the expression of Crystallin Zeta in the lens is relatively low compared to other mammalian species. The protein is expressed ubiquitously in various tissues and has been implicated in several physiological and pathological processes.

Clinical Significance

Crystallin Zeta has been associated with various diseases, including transitional papilloma and developmental and epileptic encephalopathy 87. Its role in stabilizing mRNAs encoding proteins involved in renal glutamine catabolism during metabolic acidosis suggests a potential involvement in metabolic regulation and response to cellular stress.

Recombinant Production

Recombinant human Crystallin Zeta is produced by expressing the CRYZ gene in systems such as E. coli, with the protein often fused to tags (e.g., His-tag) to facilitate purification. This recombinant protein is used in research to study its structure, function, and potential therapeutic applications.

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CRYZ Human

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