CRYAB Antibody

Crystallin Alpha B is a molecular chaperone that prevents the aggregation of misfolded proteins, thereby protecting cells from stress-induced damage. It has a molecular weight of approximately 20 kDa and forms large oligomeric complexes. The protein consists of an N-terminal domain, a conserved alpha-crystallin domain, and a C-terminal extension. The alpha-crystallin domain is responsible for its chaperone activity, while the N-terminal and C-terminal regions contribute to its oligomerization and interaction with other proteins.

Crystallin Alpha B is expressed in various tissues, with particularly high levels in the lens of the eye, where it contributes to the transparency and refractive properties of the lens. It is also expressed in the heart, skeletal muscle, brain, and other tissues. The expression of Crystallin Alpha B is regulated by various stress conditions, including heat shock, oxidative stress, and mechanical stress. It is also upregulated in response to certain pathological conditions, such as neurodegenerative diseases, cardiomyopathies, and cancer.

Crystallin Alpha B has been implicated in several diseases due to its role in protein homeostasis and cellular protection. In neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease, Crystallin Alpha B is upregulated and may help protect neurons from protein aggregation and oxidative stress. In cardiomyopathies, mutations in the CRYAB gene can lead to the development of desmin-related myopathy, a condition characterized by the accumulation of misfolded proteins and muscle weakness. Additionally, Crystallin Alpha B is overexpressed in various cancers and may contribute to tumor progression and resistance to therapy.

The Mouse Anti Human Crystallin Alpha B Antibody is a monoclonal antibody that specifically recognizes the human Crystallin Alpha B protein. This antibody is widely used in research to study the expression, localization, and function of Crystallin Alpha B in various tissues and under different conditions. It is suitable for various applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry (Flow Cyt), and immunoprecipitation (IP).

The antibody is produced by immunizing mice with the full-length human Crystallin Alpha B protein or a synthetic peptide corresponding to a specific region of the protein. The resulting hybridoma cells are screened for the production of antibodies that specifically recognize human Crystallin Alpha B. The selected monoclonal antibody is then purified and characterized for its specificity and affinity.

The Mouse Anti Human Crystallin Alpha B Antibody is a valuable tool for researchers studying the role of Crystallin Alpha B in various physiological and pathological processes. It can be used to:

• Detect and quantify Crystallin Alpha B in tissue samples and cell lysates using Western blotting.
• Visualize the localization of Crystallin Alpha B in tissues and cells using immunohistochemistry and immunocytochemistry.
• Analyze the expression of Crystallin Alpha B in different cell populations using flow cytometry.
• Investigate protein-protein interactions involving Crystallin Alpha B using immunoprecipitation.