CRTAM Human
Description
CRTAM is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CRTAM, short for Cytotoxic and Regulatory T Cell Molecule, is a protein belonging to the immunoglobulin superfamily. Structurally resembling the JAM family, it's closer to nectin-like proteins in evolutionary terms. CRTAM plays a role in the adhesion of epithelial cells. Additionally, it interacts with CADM1 and contributes to the cytotoxic activity of natural killer (NK) cells.
This CRTAM protein features an 8 amino acid His tag located at its C-terminus. Its purification is carried out through proprietary chromatographic methods.
| The CRTAM protein solution is provided at a concentration of 1mg/ml. It is formulated in a solution consisting of phosphate buffered saline at a pH of 7.4 and 10% glycerol. |
To further enhance stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA.
Repeated freezing and thawing of the product should be avoided.
Product Science Overview
The Cytotoxic and Regulatory T Cell Molecule (CRTAM) is a protein that plays a crucial role in the immune system, particularly in the regulation and activation of T cells. This molecule is encoded by the CRTAM gene in humans and is involved in various immune responses, including cytotoxicity and regulation of T cell proliferation.
CRTAM is a glycosylated polypeptide chain consisting of 278 amino acids with a molecular mass of approximately 31.0 kDa . It is expressed with an 8 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques . The protein is typically produced in Sf9 insect cells and is available in a sterile filtered colorless solution containing phosphate-buffered saline and glycerol .
CRTAM mediates heterophilic cell-cell adhesion, which is essential for the activation, differentiation, and tissue retention of various T cell subsets . It interacts with CADM1 to promote natural killer (NK) cell cytotoxicity and interferon-gamma (IFNG) secretion by CD8+ T cells in vitro . This interaction also facilitates NK cell-mediated rejection of tumors expressing CADM1 in vivo .
Additionally, CRTAM regulates CD8+ T cell proliferation in response to T cell receptor (TCR) activation and is involved in the late phase of cellular polarization of a subset of CD4+ T cells . This regulation is crucial for TCR-mediated proliferation and the production of cytokines such as IFNG, IL17, and IL22 .
The interaction between CRTAM and CADM1 is significant in the context of tumor immunity. By promoting NK cell cytotoxicity and the secretion of IFNG, CRTAM plays a role in the immune system’s ability to target and eliminate tumor cells . This makes CRTAM a potential target for therapeutic interventions in cancer treatment.
