CREB3L2 Human

CAMP Responsive Element Binding Protein 3-Like 2 Human Recombinant

Cat. No.

BT2114

Source

Escherichia Coli.

Synonyms

CAMP Responsive Element Binding Protein 3-Like 2, BBF2H7, CAMP-Responsive Element-Binding Protein 3-Like Protein 2, BBF2 Human Homolog On Chromosome 7, Cyclic AMP-Responsive Element-Binding Protein 3-Like Protein 2, Basic Transcription Factor 2, B-ZIB Transcription Factor, FUS/BBF2H7 Protein, TCAG_1951439, Cyclic AMP-responsive element-binding protein 3-like protein 2, cAMP-responsive element-binding protein 3-like protein 2.

Appearance

Sterile filtered colorless solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs

In Stock

Quick Inquiry

Description

CREB3L2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 401 amino acids (1-378 a.a) and having a molecular mass of 44kDa.

CREB3L2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

CREB3L2, also known as CAMP Responsive Element Binding Protein 3-Like 2, is a member of the oasis bZIP transcription factor family. These proteins can form homodimers but not heterodimers. CREB3L2 acts as a transcriptional activator. Translocations involving CREB3L2 on chromosome 7 and the gene fused in sarcoma on chromosome 16 have been observed in certain tumors. Myxofibrosarcoma is one disease associated with CREB3L2.

Description

Recombinant human CREB3L2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 401 amino acids (amino acids 1-378) and has a molecular weight of 44 kDa. The protein features a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless solution, sterile-filtered.

Formulation

CREB3L2 protein solution at a concentration of 0.25 mg/ml in 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, and 1mM DTT.

Stability

For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Purity exceeds 90% as determined by SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEVLESG EQGVLQWDRK LSELSEPGDG EALMYHTHFS ELLDEFSQNV LGQLLNDPFL SEKSVSMEVE PSPTSPAPLI QAEHSYSLCE EPRAQSPFTH ITSDSFNDDE VESEKWYLST DFPSTSIKTE PITDEPPPGL VPSVTLTITA ISTPLEKEEP PLEMNTGVDS SCQTIIPKIK LEPHEVDQFL NFSPKEAPVD HLHLPPTPPS SHGSDSEGSL SPNPRLHPFS LPQTHSPSRA APRAPSALSS SPLLTAPHKL QGSGPLVLTE EEKRTLIAEG YPIPTKLPLS KSEEKALKKI RRKIKNKISA QESRRKKKEY MDSLEKKVES CSTENLELRK KVEVLENTNR TLLQQLQKLQ TLVMGKVSRT CKLAGTQTGT C.

Product Science Overview

Introduction

CAMP Responsive Element Binding Protein 3-Like 2 (CREB3L2) is a member of the basic leucine zipper (bZIP) transcription factor family. This protein plays a crucial role in various cellular processes, including the unfolded protein response (UPR) and chondrogenesis. It is encoded by the CREB3L2 gene, located on chromosome 7q33 .

Gene and Protein Structure

The CREB3L2 gene consists of 12 exons and spans approximately 122,064 base pairs. The gene produces multiple transcript variants encoding different isoforms. The protein itself is composed of 519 amino acids, with a molecular weight of approximately 57 kDa. It contains several key domains:

bZIP domain: This domain is involved in DNA binding and dimerization, essential for its function as a transcription factor.
Transmembrane domain: Located between residues 376-397, this domain anchors the protein to the endoplasmic reticulum (ER) membrane.

Function

CREB3L2 is primarily involved in the unfolded protein response (UPR). Under normal conditions, it is inserted into the ER membrane, with its N-terminal DNA-binding and transcription activation domains facing the cytosol. Upon ER stress, CREB3L2 is transported to the Golgi apparatus, where it is cleaved by resident proteases. The cleaved N-terminal domain then translocates to the nucleus to activate the transcription of target genes.

In addition to its role in UPR, CREB3L2 is critical for chondrogenesis. It activates the transcription of SEC23A, a gene involved in the transport and secretion of cartilage matrix proteins.

Clinical Significance

Mutations or translocations involving the CREB3L2 gene have been implicated in various cancers, including myxofibrosarcoma and soft tissue sarcoma. The most common translocation is t(7;16)(q33;p11), which results in the fusion of CREB3L2 with the FUS gene. This fusion protein is thought to contribute to tumorigenesis by deregulating genes normally controlled by CREB3L2.

Expression

The expression of CREB3L2 is tissue-specific. It is highly expressed in the placenta, lung, spleen, and intestine, while lower levels are found in the heart, brain, skeletal muscle, thymus, colon, and leukocytes. In fetal tissues, the weakest expression is detected in the brain and heart.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Product Name

Quantity&Size*

Message

CREB3L2 Human

Description

Product Specs

Product Science Overview

Quick Inquiry

Category

Service