CRCP Human

CGRP Receptor Component Human Recombinant
Cat. No.
BT2009
Source
E.coli.
Synonyms
CGRP receptor component protein, CGRP-RCP, RCP, RCP9, Calcitonin gene-related peptide-receptor component protein, RNA polymerase III subunit C9, DNA-directed RNA polymerase III subunit RPC9, HsC17, MGC111194.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CRCP Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 168 amino acids (1-148) and having a molecular mass of 19.0 kDa.
The CRCP is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
CRCP, a ubiquitous coupling protein, plays a crucial role in the function of calcitonin gene-related peptide and adrenomedullin receptors. It regulates ligand sensitivity across various tissues and exhibits multiple activities including DNA-directed RNA polymerase activity, catalytic activity, and calcitonin receptor activity.
Description
Recombinant human CRCP, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 168 amino acids (residues 1-148). With a molecular weight of 19.0 kDa, it includes a 20 amino acid His-Tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
The CRCP solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C in a frozen state is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. It is important to avoid repeated cycles of freezing and thawing.
Purity
The purity is determined to be greater than 95% using SDS-PAGE analysis.
Synonyms
CGRP receptor component protein, CGRP-RCP, RCP, RCP9, Calcitonin gene-related peptide-receptor component protein, RNA polymerase III subunit C9, DNA-directed RNA polymerase III subunit RPC9, HsC17, MGC111194.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MEVKDANSAL LSNYEVFQLL TDLKEQRKES GKNKHSSGQQ NLNTITYETL KYISKTPCRH QSPEIVREFL TALKSHKLTK AEKLQLLNHR PVTAVEIQLM VEESEERLTE EQIEALLHTV TSILPAEPEA EQKKNTNSNV AMDEEDPA

Product Science Overview

Introduction

The Calcitonin Gene-Related Peptide (CGRP) is a neuropeptide that plays a crucial role in various physiological processes, including vasodilation and the transmission of nociceptive (pain) information. It is particularly significant in the context of migraine pathophysiology, where elevated levels of CGRP are observed during acute migraine attacks .

CGRP Receptor Complex

The CGRP receptor is a complex structure composed of multiple components:

  1. Calcitonin Receptor-Like Receptor (CLR): A seven transmembrane-spanning protein.
  2. Receptor Activity-Modifying Protein 1 (RAMP1): A single transmembrane-spanning protein.
  3. Receptor Component Protein (RCP): An intracellular protein .

These components work together to form a functional receptor that can bind to CGRP and mediate its effects.

Expression and Localization

CGRP and its receptor components are widely expressed throughout the central and peripheral nervous systems. In particular, they are found in regions involved in the transmission of pain and the regulation of blood flow. For example, in the spinal trigeminal nucleus (STN) and the C1-level of the spinal cord, CGRP and its receptor components are expressed in specific patterns that suggest their involvement in primary headaches, such as migraines .

Role in Migraine

CGRP is a key player in the pathophysiology of migraines. During a migraine attack, CGRP is released from the trigeminal vascular system, leading to vasodilation and the activation of pain pathways. This makes the CGRP receptor a promising target for the development of novel anti-migraine therapies .

Human Recombinant CGRP Receptor Component

The human recombinant CGRP receptor component is a biotechnologically engineered version of the naturally occurring receptor. It is used in research and therapeutic applications to study the receptor’s function and to develop drugs that can modulate its activity. The recombinant receptor component allows for precise control and manipulation in experimental settings, providing valuable insights into the role of CGRP in various physiological and pathological processes .

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