CPSF4 Human

Cleavage And Polyadenylation Specific Factor 4 Human Recombinant
Cat. No.
BT1866
Source
Escherichia Coli.
Synonyms
CPSF30, NAR, NEB1, Neb-1, CPSF 30 kDa subunit, NS1 effector domain-binding protein 1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CPSF4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 267 amino acids (1-244 a.a.) and having a molecular mass of 29.9kDa.
CPSF4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cleavage And Polyadenylation Specific Factor 4, also known as CPSF4, is a protein belonging to the CPSF4/YTH1 family. This protein is integral to the CPSF4 complex, which plays a crucial role in the 3'-end formation of pre-mRNA. The complex functions by identifying the AAUAAA signal sequence and interacting with poly(A) polymerase and other relevant factors to enable cleavage and the addition of a poly(A) tail. Notably, CPSF4 exhibits a binding preference for poly(U) RNA polymers.
Description
This product consists of recombinant human CPSF4 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 267 amino acids (specifically, amino acids 1 to 244) with a molecular weight of 29.9 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has been sterilized through filtration.
Formulation
The CPSF4 protein solution has a concentration of 1 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.4M UREA, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To further enhance long-term stability, consider adding a carrier protein like HSA or BSA at a concentration of 0.1%. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the CPSF4 protein is greater than 85.0%, as determined by SDS-PAGE analysis.
Synonyms
CPSF30, NAR, NEB1, Neb-1, CPSF 30 kDa subunit, NS1 effector domain-binding protein 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMQEIIAS VDHIKFDLEI AVEQQLGAQP LPFPGMDKSG AAVCEFFLKA ACGKGGMCPF RHISGEKTVV CKHWLRGLCK KGDQCEFLHE YDMTKMPECY FYSKFGECSN KECPFLHIDP ESKIKDCPWY DRGFCKHGPL CRHRHTRRVI CVNYLVGFCP EGPSCKFMHP RFELPMGTTE QPPLPQQTQP PAKQRTPQVI GVMQSQNSSA GNRGPRPLEQ VTCYKCGEKG HYANRCTKGH LAFLSGQ.

Product Science Overview

Role in mRNA Processing

CPSF4 is integral to the 3’ end processing machinery of cellular pre-mRNAs . The process of cleavage and polyadenylation involves two main steps:

  1. Endonucleolytic Cleavage: This occurs at a polyadenylation site (PAS) within the pre-mRNA.
  2. Polyadenylation: Following cleavage, a polyadenosine (poly(A)) tail is synthesized at the 3’ end of the mRNA .

These steps are regulated by both cis-regulatory elements within the pre-mRNA and trans-acting CPA machinery and associated proteins .

Clinical Significance

Recent studies have highlighted the importance of CPSF4 in cancer biology. For instance, CPSF4 has been found to be highly expressed in lung adenocarcinoma cell lines and tumor tissues, while being undetectable in normal human tissues . This overexpression is correlated with poor overall survival in patients with lung adenocarcinomas, making CPSF4 a potential prognostic biomarker and therapeutic target .

Mechanisms of Action

The biological role of CPSF4 in cancer involves multiple signaling pathways. Suppression of CPSF4 by siRNA has been shown to inhibit lung cancer cell proliferation, colony formation, and induce apoptosis . These effects are achieved through the modulation of signaling pathways such as PI3K, AKT, ERK1/2, and JNK . Additionally, CPSF4 knockdown induces the cleavage of caspase-3 and caspase-9 proteins, further promoting apoptosis .

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