CPOX Antibody
Description
Product Specs
Product Science Overview
Coproporphyrinogen oxidase (CPOX) is an enzyme that plays a crucial role in the biosynthesis of heme, an essential component of hemoproteins such as hemoglobin. The enzyme is involved in the sixth step of the heme production process, where it catalyzes the oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX . This step is vital for the proper functioning of various biological processes, including oxygen transport and cellular respiration.
CPOX is encoded by the CPOX gene in humans. Mutations in this gene can lead to a reduced production of heme, resulting in medical conditions such as hereditary coproporphyria and harderoporphyria . These conditions are characterized by the accumulation of porphyrin precursors in the body, leading to various symptoms, including abdominal pain, neurological disturbances, and photosensitivity .
Mouse anti-human antibodies are secondary antibodies produced by immunizing mice with human immunoglobulins. These antibodies are used in various research and diagnostic applications to detect, sort, or purify human proteins . They are affinity-purified to ensure high specificity and are often conjugated with labels such as horseradish peroxidase (HRP), alkaline phosphatase (AP), or fluorescent dyes to facilitate detection .
Mouse anti-human antibodies are commonly used in techniques such as Western blotting, ELISA, flow cytometry, and immunohistochemistry . These antibodies provide increased versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody .
Combining the knowledge of CPOX and mouse anti-human antibodies, Coproporphyrinogen Oxidase, Mouse Anti Human refers to the use of mouse-derived antibodies to detect human CPOX in various experimental settings. This approach is valuable in research focused on understanding the role of CPOX in heme biosynthesis and its implications in diseases such as hereditary coproporphyria.
By using mouse anti-human antibodies specific to CPOX, researchers can study the expression, localization, and activity of this enzyme in human tissues and cells. This information is crucial for developing therapeutic strategies to manage conditions associated with CPOX deficiencies.
