CPEB1 Human
Description
CPEB1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1) is a sequence-specific RNA-binding protein that plays a crucial role in the regulation of mRNA cytoplasmic polyadenylation and translation initiation. This protein is involved in various cellular processes, including oocyte maturation, early development, and synaptic plasticity in neurons .
CPEB1 binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5’-UUUUUAU-3’) within the mRNA 3’-untranslated region (3’-UTR) . This binding regulates the elongation of the polyadenine tail of messenger RNA (mRNA), which in turn influences the stability and translation of the mRNA .
CPEB1 can act as both an activator and a repressor of translation, depending on its phosphorylation state . When phosphorylated, CPEB1 promotes the elongation of the polyadenine tail, leading to the activation of translation. Conversely, when unphosphorylated, CPEB1 interacts with the deadenylation complex to shorten the polyadenine tail, thereby repressing translation .
CPEB1 is involved in several critical cellular processes:
- Oocyte Maturation: CPEB1 was first identified in Xenopus oocytes, where it regulates oocyte maturation by controlling the translation of specific mRNAs .
- Early Development: During early development, CPEB1 regulates the translation of mRNAs that are essential for cell division and differentiation .
- Synaptic Plasticity: In neurons, CPEB1 is present at postsynaptic sites and dendrites, where it regulates the translation of mRNAs in response to synaptic activity. This regulation is crucial for synaptic plasticity, learning, and memory .
CPEB1 is highly conserved across different species, indicating its fundamental role in cellular processes. The protein family to which CPEB1 belongs includes four members: CPEB1, CPEB2, CPEB3, and CPEB4. These proteins share similar functions but have distinct roles in different tissues and developmental stages .
Disruption in the function of CPEB1 has been associated with various pathologies, including autism spectrum disorder and brain cancer . Additionally, CPEB1 gene regulation has shown potential in the recovery of brain function in patients with fragile X syndrome and Huntington’s disease, making it a promising target for gene therapy .
