COX4NB Human

COX4NB Human Recombinant
Cat. No.
BT1488
Source
E.coli.
Synonyms

COX4 neighbor, COX4AL, Protein FAM158B, NOC4, C16orf2, chromosome 16 open reading frame 2, C16orf4, chromosome 16 open reading frame 4, family with sequence similarity
158-member B.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

COX4NB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 230 amino acids (1-210) and having a molecular mass of 25.9 kDa.
The COX4NB is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
COX4NB, a member of the UPF0172 (NOC4) family, resides on chromosome 16 adjacent to the COX4 gene. Its expression is highest in the pancreas, moderate in the heart, skeletal muscle, and placenta, and low in the liver, lung, kidney, and brain.
Description
Recombinant human COX4NB, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 230 amino acids (residues 1-210). It has a molecular weight of 25.9 kDa. The COX4NB protein is expressed with a 20 amino acid His-Tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The COX4NB solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of COX4NB is greater than 95% as determined by SDS-PAGE analysis.
Synonyms

COX4 neighbor, COX4AL, Protein FAM158B, NOC4, C16orf2, chromosome 16 open reading frame 2, C16orf4, chromosome 16 open reading frame 4, family with sequence similarity
158-member B.

Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPGVKLTTQA YCKMVLHGAK YPHCAVNGLL VAEKQKPRKE HLPLGGPGAH HTLFVDCIPL FHGTLALAPM LEVALTLIDS WCKDHSYVIA GYYQANERVK DASPNQVAEK VASRIAEGFS DTALIMVDNT KFTMDCVAPT IHVYEHHENR WRCRDPHHDY CEDWPEAQRI SASLLDSRSY ETLVDFDNHL DDIRNDWTNP EINKAVLHLC

Product Science Overview

Protein Structure and Expression

The recombinant human COX4NB protein consists of 225 amino acids and has a predicted molecular mass of 25.6 kDa. When analyzed under reducing conditions using SDS-PAGE, it migrates as an approximately 27 kDa band . The protein is expressed in various tissues, including the liver, pancreas, heart, lung, kidney, brain, skeletal muscle, and placenta. Expression levels are highest in the pancreas and moderate in the heart, skeletal muscle, and placenta .

Production and Purification

The recombinant human COX4NB protein is typically produced in E. coli. The protein is expressed with a polyhistidine tag (His-tag) at the N-terminus, which facilitates its purification using conventional chromatography techniques . The purity of the recombinant protein is greater than 95%, as determined by SDS-PAGE .

Stability and Storage

Recombinant human COX4NB protein is usually provided as a lyophilized powder, which is stable for up to twelve months when stored at -20°C to -80°C under sterile conditions. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles . For reconstitution, specific buffer information and instructions are provided with the product .

Biological Function

COX4NB is involved in various biological processes, although its exact functions are still being studied. It is known to be associated with the endoplasmic reticulum (ER) membrane complex and plays a role in cellular homeostasis and protein processing .

Applications

Recombinant human COX4NB protein is used in various research applications, including studies on protein-protein interactions, cellular signaling pathways, and the functional analysis of the COX4NB gene. Its high purity and stability make it a valuable tool for biochemical and structural studies .

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