COX4 neighbor, COX4AL, Protein FAM158B, NOC4, C16orf2, chromosome 16 open reading frame 2, C16orf4, chromosome 16 open reading frame 4, family with sequence similarity
158-member B.
COX4 neighbor, COX4AL, Protein FAM158B, NOC4, C16orf2, chromosome 16 open reading frame 2, C16orf4, chromosome 16 open reading frame 4, family with sequence similarity
158-member B.
The recombinant human COX4NB protein consists of 225 amino acids and has a predicted molecular mass of 25.6 kDa. When analyzed under reducing conditions using SDS-PAGE, it migrates as an approximately 27 kDa band . The protein is expressed in various tissues, including the liver, pancreas, heart, lung, kidney, brain, skeletal muscle, and placenta. Expression levels are highest in the pancreas and moderate in the heart, skeletal muscle, and placenta .
The recombinant human COX4NB protein is typically produced in E. coli. The protein is expressed with a polyhistidine tag (His-tag) at the N-terminus, which facilitates its purification using conventional chromatography techniques . The purity of the recombinant protein is greater than 95%, as determined by SDS-PAGE .
Recombinant human COX4NB protein is usually provided as a lyophilized powder, which is stable for up to twelve months when stored at -20°C to -80°C under sterile conditions. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles . For reconstitution, specific buffer information and instructions are provided with the product .