Protein is >95% pure as determined by 12% SDS-PAGE (coomassie staining).
The Recombinant Human Coronavirus 229E, E.Coli derived, 359 amino acids, contains the nucleocapsid immunodominant regions. The protein is fused to a 6xHis tag at C-terminal and purified by standard chromatography techniques.
Recombinant Human Coronavirus 229E, produced in E. coli, is a 359 amino acid protein containing the nucleocapsid's immunodominant regions. A 6xHis tag is attached to the protein's C-terminal, and it undergoes purification using standard chromatographic methods.
SDS-PAGE analysis (using Coomassie staining) indicates that the protein's purity is greater than 95%.
CoV-229E (0.88mg/ml) is supplied in a solution of PBS with 25mM K2CO3.
P RNLVPINKKD KNKLIGYWNV QKRFRTRKGK RVDLSPKLHF YYLGTGPHKD AKFRERVEGV VWVAVDGAKT EPTGYGVRRK NSEPEIPHFN QKLPNGVTVV EEPDSRAPSR SQSRSQSRGR GESKPQSRNP SSDRNHNSQD DIMKAVAAAL KSLGFDKPQE KDKKSAKTGT PKPSRNQSPA SSQTSAKSLA RSQSSETKEQ KHEMQKPRWK RQPNDDVTSN VTQCFGPRDL DHNFGSAGVV ANGVKAKGYP QFAELVPSTA AMLFDSHIVS KESGNTVVLT FTTRVTVPKD HPHLGKFLEE LNAFTREMQQ
Human coronavirus 229E (HCoV-229E) is one of the seven known coronaviruses that infect humans. It was first identified in 1965 by Dorothy Hamre at the University of Chicago . HCoV-229E is a member of the Alphacoronavirus genus and is known to cause mild upper respiratory tract infections, commonly referred to as the common cold .
HCoV-229E has a positive-sense single-stranded RNA genome. The virus encodes several nonstructural proteins (nsps) that play crucial roles in its replication and transcription. One of these proteins, nonstructural protein 8 (nsp8), has been shown to have metal ion-dependent RNA 3’-terminal adenylyltransferase (TATase) activity . This activity is essential for viral replication and is conserved among coronaviruses .
Recombinant forms of HCoV-229E are engineered versions of the virus used for research purposes. These recombinant viruses are typically modified to express specific proteins or to study the functions of viral components. For example, recombinant HCoV-229E nsp8 has been characterized to understand its role in viral replication .
HCoV-229E is generally associated with mild respiratory illnesses. However, it can cause severe infections in infants, elderly individuals, and immunocompromised patients . Understanding the molecular mechanisms of HCoV-229E, including the functions of its recombinant forms, is crucial for developing antiviral therapies and vaccines.