SARS Membrane
Protein is >90% pure as determined SDS-PAGE.
Description
The E.coli derived recombinant protein contains the Membrane/Matrix protein immunodominant regions fused to 6xHis tag at C-terminal.
Product Specs
The membrane protein of the SARS virus is crucial for its life cycle. This protein includes three structural antigens found on the outer cell surface: the envelope (E), membrane (M), and spike (S) antigens. The SARS membrane protein is essential for the virus's assembly, budding, envelope formation, and ability to cause disease.
This recombinant protein is derived from E. coli bacteria and contains the immunodominant regions of the SARS Membrane/Matrix protein. It has a 6xHis tag fused to its C-terminal end.
The SARS Membrane protein solution is supplied in phosphate-buffered saline (PBS).
The protein is shipped with ice packs to keep it cold. Upon arrival, it should be stored at -20 degrees Celsius.
SDS-PAGE analysis indicates that the protein is greater than 90% pure.
Purified by proprietary chromatographic technique.
Immunoreactive with sera of SARS-infected individuals.
Product Science Overview
The Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) emerged in 2002 in Foshan City, Guangdong Province, China, and caused a global health crisis due to its high transmissibility and severe health impacts . The SARS-CoV is a member of the Coronaviridae family and has been identified as an interspecies-transmissible virus .
The membrane (M) protein of SARS-CoV is one of the major structural proteins of the virus, along with the spike (S), envelope (E), and nucleocapsid (N) proteins . The M protein is the most abundant structural protein in the virus and plays a crucial role in virus assembly and morphogenesis . It is composed of a triple membrane-spanning domain, a short N-terminus that protrudes out of the membrane, and a long cytoplasmic C-terminus .
The M protein has been identified as a dominant immunogen, meaning it can stimulate a strong immune response . It has been shown to elicit both humoral (antibody-mediated) and cellular (T-cell-mediated) immune responses . This makes the M protein a potential target for vaccine development against SARS-CoV .
Recombinant M protein refers to the M protein that has been produced using recombinant DNA technology. This involves inserting the gene encoding the M protein into a suitable expression system, such as bacteria, yeast, or mammalian cells, to produce the protein in large quantities. Recombinant M protein can be used in various applications, including vaccine development, diagnostic assays, and research studies to understand the structure and function of the M protein .
