COPZ1 Human

Coatomer Protein Complex, Subunit Zeta 1 Human Recombinant
Cat. No.
BT1342
Source
Escherichia Coli.
Synonyms
Coatomer protein complex subunit zeta 1, Zeta-1 COP, CGI-120, COPZ.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

COPZ1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 197 amino acids (1-177a.a.) and having a molecular mass of 22.3kDa.
The COPZ1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
COPZ1, a member of the adaptor complexes small subunit family, constitutes a subunit of the coatomer complex. This complex plays a crucial role in biosynthetic protein transport. COPZ1, specifically, contributes to the regulation of coat assembly and influences the rate of protein transport through its dynamic association and dissociation with the coatomer complex.
Description
Recombinant COPZ1, of human origin, is expressed in E. coli and fused with a 20 amino acid His tag at its N-terminus. This protein is a non-glycosylated polypeptide chain composed of 197 amino acids (residues 1-177), resulting in a molecular weight of 22.3 kDa. Purification of COPZ1 is achieved using proprietary chromatographic methods.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for clarity.
Formulation
The COPZ1 solution is supplied at a concentration of 1 mg/ml and is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 10% glycerol, 1 mM DTT, and 0.1 M NaCl.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To further enhance long-term stability, consider adding a carrier protein such as HSA or BSA (0.1%). Repeated freezing and thawing of the product should be avoided.
Purity
The purity of COPZ1 is determined by SDS-PAGE analysis to be greater than 95.0%.
Synonyms
Coatomer protein complex subunit zeta 1, Zeta-1 COP, CGI-120, COPZ.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MEALILEPSL YTVKAILILD NDGDRLFAKY YDDTYPSVKE QKAFEKNIFN KTHRTDSEIA LLEGLTVVYK SSIDLYFYVI GSSYENELML MAVLNCLFDS LSQMLRKNVE KRALLENMEG LFLAVDEIVD GGVILESDPQ QVVHRVALRG EDVPLTEQTV SQVLQSAKEQ IKWSLLR.

Product Science Overview

Structure and Function

The coatomer complex binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. This association mediates biosynthetic protein transport from the endoplasmic reticulum (ER), through the Golgi apparatus, and up to the trans-Golgi network . The zeta subunit, COPZ1, is particularly involved in regulating the coat assembly and the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex .

Gene and Protein Information

The COPZ1 gene is located on chromosome 12 and is a protein-coding gene. It has several aliases, including CGI-120, COPZ, and HSPC181 . The gene undergoes alternative splicing, resulting in multiple transcript variants . The protein encoded by COPZ1 is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins .

Biological Pathways

COPZ1 is involved in several critical biological pathways, including:

  • Transport to the Golgi and subsequent modification
  • wtCFTR and delta508-CFTR traffic / Generic schema (norm and CF)
Expression and Localization

COPZ1 is expressed in various tissues, including lymphoid tissue, bone marrow, testis, and skeletal muscle . It is involved in processes such as cell proliferation, innate immune response, protein ubiquitination, and lymph vessel development .

Clinical Significance

Mutations or dysregulation of COPZ1 can have significant implications for cellular function and may be associated with various diseases. The coatomer complex, including COPZ1, is essential for the proper functioning of cellular transport mechanisms, and any disruption can lead to cellular dysfunction .

Research and Applications

Human recombinant COPZ1 is used in research to study its role in intracellular protein trafficking and autophagy. It is also utilized in various assays and experiments to understand its function and regulation within the cell .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.