Collagen-V Bovine
Bovine placenta.
Filtered White lyophilized (freeze-dried) powder.
> 98.0%.
Description
Bovine Collagen-V is a natural protein purified from bovine placenta. Collagen-V is purified by proprietary chromatographic techniques.
Product Specs
As a vital part of the extracellular matrix, collagen is a fibrous protein that gives tissues their structural integrity by providing tensile strength. Collagen and its derivative, gelatin, have been extensively utilized in medical, pharmaceutical, and consumer products for over a century. These materials, derived from animal remains, are readily available and cost-effective. However, most formulations lack high purity levels and may trigger inflammatory reactions in certain users. Moreover, concerns have arisen in recent years regarding the potential contamination of bovine products with the infectious agent responsible for mad cow disease and its human counterpart, Creutzfeldt-Jakob Disease. Animal-derived collagens undergo significant modifications throughout their lifespan in the extracellular space, influencing their extractability from tissues and biophysical properties. Consequently, collagens extracted from tissues exhibit considerable lot-to-lot variability and, as bulk materials, often pose analytical challenges. Products containing animal-derived collagen can potentially elicit adverse inflammatory or immune responses in humans and carry the risk of contamination with viruses or prions, which are potentially life-threatening pathogens. Recombinant collagens, being nearly identical to the native collagen protein, minimize the risks of inflammation, immune reactions, and disease compared to their animal-sourced counterparts.
Collagen-V, of bovine origin, is a naturally occurring protein extracted from bovine placenta. The purification process of Collagen-V involves proprietary chromatographic techniques.
Collagen-V presents as a white, lyophilized (freeze-dried) powder that has been filtered.
The lyophilization process of Collagen-V was carried out without the addition of any other substances.
For reconstitution of the lyophilized Collagen-V, it is advisable to use 20 mM acetic acid at a concentration of at least 1mg/ml. Subsequently, further dilutions can be made using other aqueous solutions.
Lyophilized Collagen-V, while stable at room temperature for up to 3 weeks, should be stored in a dry environment below -18°C. After reconstitution, Collagen-V should be stored at 4°C for 2-7 days. For future use, it should be stored below -18°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid subjecting the product to repeated freeze-thaw cycles.
The purity of Collagen-V exceeds 98.0%.
Bovine placenta.
Product Science Overview
Collagen is the most abundant protein in the animal kingdom, playing a crucial role in the structural integrity of various tissues. Among the different types of collagen, Type V collagen (Col. V) is a quantitatively minor fibrillar collagen that is essential for the proper assembly and function of other collagen types, particularly Type I collagen . Bovine Collagen-V, derived from cows, has garnered significant attention due to its unique properties and potential applications in medical and cosmetic fields.
Bovine Collagen-V consists of three different chains: α-1, α-2, and α-3. Each chain contains two propeptides, P5NP (N-terminal) and P5CP (C-terminal), which include signal peptides, a thrombospondin domain or von Willebrand factor C domain, and a non-collagenous domain . This complex structure allows Col. V to interact with various molecules such as DNA, heparan sulfate, thrombospondin, heparin, and insulin .
Bovine Collagen-V is commonly extracted from connective tissues such as skin, cornea, skeletal muscle, and placenta . The extraction process typically involves acid-enzymatic hydrolysis combined with ultrafiltration to achieve high purity. For instance, Col. V can be purified from the bovine cornea, resulting in a product with over 90% purity as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance liquid chromatography .
Col. V plays a pivotal role in the initiation of collagen fibril assembly and regulates the diameter of Col. I and Col. III fibrils . It is essential for the proper fibrillation of Col. I, which is the most abundant collagen type in the body . Additionally, Col. V has been shown to influence cell behaviors, such as inhibiting fibroblast proliferation and promoting cell migration .
Due to its unique properties, Bovine Collagen-V has potential applications in various fields. In regenerative medicine, it can be used to develop scaffolds for tissue engineering and wound healing. In the cosmetic industry, Col. V is utilized in anti-aging products to improve skin elasticity and reduce wrinkles .
