Collagen-IV Bovine

Collagen is a fibrous protein that forms the structural basis of various tissues, offering tensile strength and maintaining structural integrity. It represents a major constituent of the extracellular matrix. Collagen and its derivative, gelatin, have found widespread use in medical, pharmaceutical, and consumer products for over a century. These materials, derived from animal sources, are readily available and cost-effective. However, many collagen formulations lack high purity levels and may trigger inflammatory reactions in certain individuals. Moreover, concerns regarding potential contamination of bovine-derived products with prions, such as the agent responsible for mad cow disease and its human counterpart, Creutzfeldt-Jakob disease, have emerged in recent years. Animal-derived collagens undergo extensive post-translational modifications throughout their lifespan in the extracellular matrix. These modifications affect both the extractability of collagen from tissues and their biophysical properties. Consequently, commercially available collagens extracted from animal tissues often exhibit significant batch-to-batch variability. Furthermore, these bulk materials can be challenging to analyze. Products containing animal-derived collagen carry the risk of eliciting adverse inflammatory or immune reactions in humans. There is also a potential risk of contamination with viruses or prions, which can lead to serious health complications. In contrast, recombinant collagens are virtually identical to their native counterparts, effectively minimizing the risks of inflammation, immune reactions, and disease transmission associated with animal-derived collagen.

Bovine Collagen-IV is a naturally occurring protein extracted from bovine placenta. This collagen type is purified using proprietary chromatographic methods.

This product appears as a white lyophilized (freeze-dried) powder that has been filtered.

Collagen-IV undergoes lyophilization without any additional ingredients.

For reconstitution of the lyophilized Collagen-IV, it is advisable to dissolve it in 20 mM acetic acid at a concentration of at least 1 mg/mL. Further dilutions can be made using other aqueous solutions.

Lyophilized Collagen-IV demonstrates stability at room temperature for a period of 3 weeks; however, it is recommended to store it in a dry environment below -18°C. Upon reconstitution, Collagen-IV can be stored at 4°C for 2 to 7 days. For extended storage, it is recommended to store it below -18°C. To ensure optimal long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. It is important to avoid repeated freeze-thaw cycles.

Greater than 90.0% pure.

Bovine placenta.