CNRIP1 Human

Cannabinoid Receptor Interacting Protein 1 Human Recombinant

Cat. No.

BT796

Source

E.coli.

Synonyms

Cannabinoid Receptor Interacting Protein 1, Cannabinoid Receptor CB1-Interacting Protein 1, Chromosome 2 Open Reading Frame 32, C2orf32, CRIP1, CRIP1a, CRIP1b, DKFZP566K1924.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

CNRIP1 Human Recombinant produced in E. coli is a single polypeptide chain containing 187 amino acids (1-164) and having a molecular mass of 21.0 kDa.
CNRIP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

CB1 cannabinoid receptor-interacting protein 1 (CNRIP1), also known as cannabinoid receptor-interacting protein 1, is a protein that in humans is encoded by the CNRIP1 gene. CNRIP1 is a member of the CNRIP family and interacts with the C-terminal tail of cannabinoid receptor 1. This interaction suggests a role for CNRIP1 in the regulation of cannabinoid receptor 1 signaling. CNRIP1 is expressed in various tissues, including the brain and testis. Its involvement in appetite, synaptic plasticity, neuroprotection, and analgesia makes it a potential target for therapeutic interventions.

Description

Recombinant CNRIP1 Human is a single polypeptide chain containing 187 amino acids (1-164) with a molecular weight of 21.0 kDa. This protein is produced in E. coli and contains a 23 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless liquid solution, sterile filtered.

Formulation

The CNRIP1 protein solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 1mM DTT, and 20% glycerol.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is advised for long-term storage. Repeated freezing and thawing of the product should be avoided.

Purity

The purity of the CNRIP1 protein is determined to be greater than 90% as analyzed by SDS-PAGE.

Synonyms

Cannabinoid Receptor Interacting Protein 1, Cannabinoid Receptor CB1-Interacting Protein 1, Chromosome 2 Open Reading Frame 32, C2orf32, CRIP1, CRIP1a, CRIP1b, DKFZP566K1924.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGDLPGL VRLSIALRIQ PNDGPVFYKV DGQRFGQNRT IKLLTGSSYK VEVKIKPSTL QVENISIGGV LVPLELKSKE PDGDRVVYTG TYDTEGVTPT KSGERQPIQI TMPFTDIGTF ETVWQVKFYN YHKRDHCQWG SPFSVIEYEC KPNETRSLMW VNKESFL

Product Science Overview

Introduction

Cannabinoid Receptor Interacting Protein 1 (CNRIP1), also known as CRIP1, is a protein that interacts with the C-terminal tail of the cannabinoid receptor 1 (CB1). This interaction plays a crucial role in modulating the signaling pathways of CB1 receptors, which are part of the endocannabinoid system. The endocannabinoid system is involved in various physiological processes, including pain sensation, mood regulation, and appetite control.

Gene and Protein Structure

The CNRIP1 gene is located on chromosome 2 and encodes a protein that has two known isoforms. These isoforms differ in their ability to suppress CB1 receptor-mediated tonic inhibition of voltage-gated calcium channels. Isoform 1 suppresses this inhibition, while Isoform 2 does not.

Function and Mechanism

CNRIP1 interacts specifically with the CB1 receptor, which is a G protein-coupled receptor (GPCR) predominantly found in the central nervous system. The interaction between CNRIP1 and CB1 is essential for the regulation of CB1 receptor signaling. This regulation is crucial for maintaining the balance of neurotransmitter release and neuronal excitability.

In the hippocampus, CNRIP1 modulates CB1 receptor activity by enhancing cannabinoid-induced G protein activation. This modulation extends the depression of excitatory currents by cannabinoids in pyramidal neurons, thereby playing a role in controlling excessive neuronal activity.

Clinical Significance

Alterations in the expression of CNRIP1 have been associated with various neurological conditions. For instance, changes in hippocampal CRIP1a levels have been observed following limbic seizures, indicating a potential role in epilepsy. Additionally, CNRIP1 has been linked to Hodgkin’s Lymphoma, Mixed Cellularity.

Research and Applications

Research on CNRIP1 is ongoing, with studies focusing on its role in modulating CB1 receptor signaling and its potential therapeutic applications. Understanding the interaction between CNRIP1 and CB1 receptors could lead to the development of new treatments for neurological disorders, including epilepsy, neurodegeneration, and mood disorders.

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CNRIP1 Human

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