CMPK1 Human

CMPK1 is a cytosolic enzyme encoded by the CMPK1 gene located on chromosome 1p33 . The human recombinant form of CMPK1 is typically expressed in HEK293T cells and purified through affinity chromatography . The enzyme has a molecular weight of approximately 25.7 kDa and is composed of 214 amino acids .

The primary function of CMPK1 is to maintain the pyrimidine nucleotide pool within the cell. It achieves this by phosphorylating CMP, UMP, and dCMP to their respective diphosphate forms . This activity is essential for the synthesis of RNA and DNA, as well as for the activation of pyrimidine analogs, which are used as anti-cancer and anti-viral drugs .

CMPK1’s enzymatic activity is regulated by ATP and magnesium ions, which are necessary for its catalytic function . The enzyme undergoes substrate-induced conformational changes that facilitate the transfer of the phosphate group .

CMPK1 is of particular interest in the field of pharmacology due to its role in the activation of pyrimidine analogs. These analogs are used in chemotherapy and antiviral treatments, making CMPK1 a potential target for drug development . Additionally, mutations or dysregulation of CMPK1 can impact nucleotide metabolism and have been associated with various diseases .

Recombinant CMPK1 is widely used in research to study nucleotide metabolism and to develop new therapeutic strategies. The enzyme’s activity can be measured using various biochemical assays, and its structure can be analyzed through techniques such as X-ray crystallography and NMR spectroscopy .