Introduction
Carboxymethylenebutenolidase homolog (CMBL), a cysteine hydrolase belonging to the dienelactone hydrolase family, exhibits high expression levels in the liver cytosol. Functioning as the human counterpart of Pseudomonas dienelactone hydrolase, an enzyme involved in the bacterial degradation pathway of halocatechol, CMBL demonstrates a preference for cleaving cyclic esters. Notably, CMBL plays a crucial role in activating medoxomil-ester prodrugs, characterized by the medoxomil moiety linked through an oxygen atom. This enzyme is susceptible to inhibition by PCMB (p-chloromercuribenzoate) and is encoded by a gene located on human chromosome 5p15.2. Moreover, CMBL facilitates the conversion of the prodrug olmesartan medoxomil into its pharmacologically active form, olmesartan, an angiotensin receptor blocker, within the liver and intestine. The enzyme's activity extends to the activation of beta-lactam antibiotics such as faropenem medoxomil and lenampicillin. CMBL exhibits widespread expression, with the highest levels detected in the liver, followed by the kidney, small intestine, and colon.
Physical Appearance
A clear and sterile solution.
Formulation
The solution contains 1mg/ml of CMBL antibody in a buffer consisting of PBS at pH 7.4 and 0.1% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For extended periods, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The CMBL antibody has undergone rigorous testing through ELISA and Western blot analysis to confirm its specificity and reactivity. However, due to variations in applications, it is recommended to titrate the reagent in each specific experiment to achieve optimal outcomes. For Western blot analysis, a dilution range of 1:500 to 1:5000 is suggested, with a starting dilution of 1:500.
Synonyms
Carboxymethylenebutenolidase homolog, CMBL, JS-1.
Purification Method
CMBL antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human CMBL mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CMBL 1-245 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and lambda light chain.
