CLNS1A Human

Chloride Channel, Nucleotide-Sensitive, 1A Human Recombinant
Cat. No.
BT7858
Source
Escherichia Coli.
Synonyms
CLNS1A, CLCI, ICLN, CLNS1B, Methylosome subunit pICln, Chloride channel, nucleotide sensitive 1A, Chloride conductance regulatory protein ICln, Chloride ion current inducer protein, Reticulocyte pICln.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CLNS1A Human Recombinant produced in E. coli is a single polypeptide chain containing 261 amino acids (1-237) and having a molecular mass of 28.8kDa (molecular size on SDS-PAGE will appear higher).
CLNS1A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Methylosome subunit pICln (CLNS1A) is a protein belonging to the pICln (TC 1.A.47) family. It is involved in various regulatory processes within cells. CLNS1A interacts with Sm proteins, hindering their assembly on U RNA and affecting snRNP biogenesis. It also inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. CLNS1A is associated with the plasma membrane, where it acts as a chloride presence regulator.
Description
Recombinant human CLNS1A, expressed in E. coli, is a single polypeptide chain comprising 261 amino acids (residues 1-237). It has a molecular weight of 28.8 kDa. However, on SDS-PAGE, it may appear larger. The protein is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CLNS1A solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 10% glycerol, and 2 mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
CLNS1A, CLCI, ICLN, CLNS1B, Methylosome subunit pICln, Chloride channel, nucleotide sensitive 1A, Chloride conductance regulatory protein ICln, Chloride ion current inducer protein, Reticulocyte pICln.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSFLKS FPPPGPAEGL LRQQPDTEAV LNGKGLGTGT LYIAESRLSW LDGSGLGFSL EYPTISLHAL SRDRSDCLGE HLYVMVNAKF EEESKEPVAD EEEEDSDDDV EPITEFRFVP SDKSALEAMF TAMCECQALH PDPEDEDSDD YDGEEYDVEA HEQGQGDIPT FYTYEEGLSH LTAEGQATLE RLEGMLSQSV SSQYNMAGVR TEDSIRDYED GMEVDTTPTV AGQFEDADVD H.

Product Science Overview

Introduction

Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A), also known as ICln, is a protein-coding gene that plays a crucial role in various cellular processes. This protein is involved in the regulation of chloride ion transport and volume regulation in cells. It is encoded by the CLNS1A gene located on chromosome 11q14.1 .

Gene and Protein Structure

The CLNS1A gene encodes a protein that consists of 237 amino acids and has a molecular mass of approximately 26.2 kDa . The protein contains four putative transmembrane domains, which are essential for its function as a chloride channel . The gene is expressed in various human tissues, including ocular ciliary epithelial cells and leukocytes .

Biological Functions

CLNS1A is involved in multiple regulatory pathways and performs diverse functions, including:

  • Regulation of small nuclear ribonucleoprotein (snRNP) biosynthesis: CLNS1A acts as a chaperone that regulates the assembly of spliceosomal U1, U2, U4, and U5 snRNPs, which are essential for the splicing of cellular pre-mRNAs .
  • Platelet activation and cytoskeletal organization: The protein complexes with numerous cytosolic proteins to perform these functions .
  • Chloride current regulation: CLNS1A is associated with the plasma membrane, where it functions as a chloride current regulator .
Expression Patterns and Tissue Distribution

The expression of CLNS1A has been observed in a variety of human tissues. Northern blot analysis has shown that the gene is expressed as an approximately 1.7-kb message in tissues such as ocular ciliary epithelial cells and leukocytes . The protein is also found in red blood cell membranes, where it forms stable complexes with beta-actin .

Regulatory Mechanisms

CLNS1A functions as a substrate adaptor in a trimeric complex with catalytic PRMT5 and MEP50 for arginine dimethylation of spliceosomal Sm proteins . This complex is predominantly localized in the nuclei of HeLa cells . Additionally, CLNS1A interacts with RIOK1, another adaptor protein, to recruit specific substrate proteins to the PRMT5 methyltransferase complex .

Modes of Action

The protein’s mode of action involves its role as a chloride channel and its regulatory functions in various cellular processes. CLNS1A is involved in chloride transport and volume regulation in red blood cells . It also plays a role in the swelling-induced chloride current (ICl-swell) in HEK293 Phoenix cells .

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