CLEC7A Human

C-Type Lectin Domain Family 7, Member A Human Recombinant
Cat. No.
BT22853
Source

Sf9, Baculovirus cells.

Synonyms

BGR, Dendritic Cell-Associated C-Type Lectin-1, Dendritic Cell-Associated C-Type Lectin 1, C-Type Lectin Domain Family 7, Member A, Lectin-Like Receptor 1, CD369 Antigen, CANDF4, SCARE2, CD369, C-Type Lectin Domain Containing 7A, C-Type Lectin Domain Family 7 Member A, C-Type (Calcium Dependent, Carbohydrate-Recognition Domain) Lectin, Superfamily Member 12, C-Type Lectin Superfamily Member 12, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, Dectin-1, CLECSF12, DECTIN1.

Appearance
Sterile Filtered clear solution.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CLEC7A Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 183 amino acids (71-244 a.a) and having a molecular mass of 21kDa. CLEC7A is fused to a 9 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
C-type lectin domain family 7 member A 1, also known as CLEC7A, is a protein that plays a crucial role in the innate immune system's defense against fungal pathogens. This protein is found on various immune cells, including monocytes, macrophages, neutrophils, dendritic cells, and T cells. Macrophages can increase their CLEC7A levels upon stimulation with GM-CSF, IL-4, or IL-13, while factors such as dexamethasone, IL-10, and LPS can decrease its expression.
Description
Recombinant human CLEC7A, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. It consists of 183 amino acids (spanning from residues 71 to 244), resulting in a molecular weight of 21 kDa. This CLEC7A protein has a 9 amino acid His-tag fused at its C-terminus to facilitate purification, which is achieved through proprietary chromatographic methods.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The CLEC7A solution is provided at a concentration of 0.5 mg/mL in a buffer consisting of phosphate-buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the CLEC7A solution should be stored at 4°C. For longer storage, it is recommended to freeze the solution at -20°C. To further enhance stability during long-term storage, adding a carrier protein like HSA or BSA to a final concentration of 0.1% is advised. It is important to avoid repeated cycles of freezing and thawing the solution.
Purity
The purity of the CLEC7A protein is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms

BGR, Dendritic Cell-Associated C-Type Lectin-1, Dendritic Cell-Associated C-Type Lectin 1, C-Type Lectin Domain Family 7, Member A, Lectin-Like Receptor 1, CD369 Antigen, CANDF4, SCARE2, CD369, C-Type Lectin Domain Containing 7A, C-Type Lectin Domain Family 7 Member A, C-Type (Calcium Dependent, Carbohydrate-Recognition Domain) Lectin, Superfamily Member 12, C-Type Lectin Superfamily Member 12, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, Dectin-1, CLECSF12, DECTIN1.

Source

Sf9, Baculovirus cells.

Amino Acid Sequence

ADPRHNSGRN PEEKDNFLSR NKENHKPTES SLDEKVAPSK ASQTTGGFSQ SCLPNWIMHG KSCYLFSFSG NSWYGSKRHC SQLGAHLLKI DNSKEFEFIE SQTSSHRINA FWIGLSRNQS EGPWFWEDGS AFFPNSFQVR NTVPQESLLH NCVWIHGSEV YNQICNTSSY SICEKELHHH HHH.

Product Science Overview

Structure and Function

CLEC7A is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain containing a partial immunoreceptor tyrosine-based activation motif (ITAM) . This structure allows CLEC7A to function as a pattern-recognition receptor (PRR) for β-1,3-linked and β-1,6-linked glucans from fungi and plants .

Role in the Immune System

CLEC7A plays a crucial role in the innate immune response by recognizing and binding to these glucans, which are components of fungal cell walls . Upon binding, CLEC7A triggers various immune responses, including phagocytosis, production of reactive oxygen species, and cytokine production . This makes it an essential player in the body’s defense against fungal pathogens .

Expression

The expression of CLEC7A is primarily found on myeloid dendritic cells, monocytes, macrophages, and B cells . It is also expressed in peripheral blood leukocytes, with higher expression levels in spleen and peripheral blood leukocytes .

Clinical Significance

Mutations or deficiencies in CLEC7A have been associated with increased susceptibility to fungal infections such as aspergillosis and familial candidiasis . This highlights the importance of CLEC7A in maintaining immune system integrity and protecting against fungal pathogens.

Research and Applications

Human recombinant CLEC7A is used in research to study its role in immune responses and to develop potential therapeutic interventions for fungal infections . Understanding the mechanisms of CLEC7A can lead to better treatments and preventive measures for fungal diseases.

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