CLEC4M Human

C-type Lectin Domain Family 4, Member M (CLEC4M), also known as DC-SIGNR, is a type II transmembrane protein that functions as a pathogen-recognition receptor. Primarily expressed in the liver and lymph nodes, CLEC4M plays a crucial role in immune surveillance by recognizing and binding to specific carbohydrate structures on the surface of pathogens. This binding facilitates the internalization and destruction of pathogens through lysosomal degradation. CLEC4M exhibits broad specificity, recognizing a diverse range of pathogens including mycobacteria, viruses like Ebola, hepatitis C, influenza A, HIV-1, West Nile virus, and the SARS-CoV acute respiratory syndrome coronavirus, and interacting with host molecules like ICAM3.

This product consists of the extracellular domain of human CLEC4M, spanning amino acids 72 to 399, fused to a C-terminal 239 amino acid hIgG-His-Tag. This recombinant protein is expressed in Sf9 insect cells using a baculovirus expression system. The result is a glycosylated polypeptide with a molecular weight of 64.8 kDa. Following expression, the protein undergoes purification using proprietary chromatographic methods to ensure high purity.

The purified CLEC4M protein solution appears colorless and is sterile-filtered.

The CLEC4M protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution is formulated in Phosphate-Buffered Saline (PBS) at a pH of 7.4 and supplemented with 10% glycerol to enhance protein stability.

For short-term storage (up to 2-4 weeks), the CLEC4M solution can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein like HSA or BSA to a final concentration of 0.1% is advised. It is crucial to avoid repeated freezing and thawing of the protein solution to maintain its integrity and activity.

The purity of the CLEC4M protein is determined using SDS-PAGE analysis and is confirmed to be greater than 95%.

CD209 antigen-like protein 1, DC-SIGN-related protein, Dendritic cell-specific ICAM-3-grabbing non-integrin 2, Liver/lymph node-specific ICAM-3-grabbing non-integrin, DC-SIGNR, DC-SIGN2, L-SIGN, CD299, CLEC4M, CD209L, CD209L1, CD299, HP10347

Sf9, Baculovirus cells.

ADPVSKVPSS LSQEQSEQDA IYQNLTQLKA AVGELSEKSK LQEIYQELTQ LKAAVGELPE KSKLQEIYQE LTRLKAAVGE LPEKSKLQEI YQELTRLKAA VGELPEKSKL QEIYQELTRL KAAVGELPEK SKLQEIYQEL TELKAAVGEL PEKSKLQEIY QELTQLKAAV GELPDQSKQQ QIYQELTDLK TAFERLCRHC PKDWTFFQGN CYFMSNSQRN WHDSVTACQE VRAQLVVIKT AEEQNFLQLQ TSRSNRFSWM GLSDLNQEGT WQWVDGSPLS PSFQRYWNSG EPNNSGNEDC AEFSGSGWND NRCDVDNYWI CKKPAACFRD ELEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH