CLEC10A Human

C-Type Lectin Domain Family 10, Member A Human Recombinant

Cat. No.

BT21486

Source

Sf9, Insect cells.

Synonyms

C-Type Lectin Domain Containing 10A, C-Type Lectin Domain Family 10 Member A, C-Type (Calcium Dependent, Carbohydrate-Recognition Domain) Lectin, Superfamily Member 14 (Macrophage-Derived), Macrophage Lectin 2 (Calcium Dependent), CLECSF13, CLECSF14, HML, C-Type (Calcium Dependent, Carbohydrate-Recognition Domain) Lectin, Superfamily Member 13 (Macrophage-Derived), C-Type Lectin Domain Family 10, Member A, C-Type Lectin Superfamily Member 14, Macrophage Lectin 2, CD301 Antigen, CD301, HML2, MGL.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs

In Stock

Quick Inquiry

Description

CLEC10A Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 241 amino acids (61-292a.a.) and having a molecular mass of 27.3kDa. (Molecular size on SDS-PAGE under reducing conditions 28-40kDa).
CLEC10A is expressed with a 9 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction

C-Type Lectin Domain Family 10, Member A (CLEC10A), also known as CD301 or macrophage galactose-type C-type lectin (MGL), is a protein that in humans is encoded by the CLEC10A gene. CLEC10A is a type II transmembrane protein belonging to the C-type lectin superfamily. It is primarily expressed on immature myeloid dendritic cells and alternatively activated macrophages. This lectin plays a role in regulating both innate and adaptive immune responses. CLEC10A exhibits calcium-dependent binding to terminal galactose and N-acetylgalactosamine residues linked to serine or threonine.

Description

Recombinant human CLEC10A protein was expressed in Sf9 insect cells. It is a single, glycosylated polypeptide chain containing 241 amino acids (corresponding to amino acid residues 61-292 of the full-length protein). The recombinant protein has a molecular mass of 27.3 kDa, determined by its amino acid sequence. On SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 28-40 kDa. The CLEC10A protein contains a 9 amino acid His-tag at the C-terminus to facilitate purification. The protein was purified using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless, and sterile-filtered solution.

Formulation

The CLEC10A protein is supplied in a solution at a concentration of 0.5 mg/ml in Phosphate Buffered Saline (pH 7.4).

Stability

For short-term storage (2-4 weeks), the protein solution should be stored at 4°C. For long-term storage, the protein solution should be stored at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

The purity of the CLEC10A protein is greater than 90% as determined by SDS-PAGE analysis.

Synonyms

Source

Sf9, Insect cells.

Amino Acid Sequence

ADPQNSKFQR DLVTLRTDFS NFTSNTVAEI QALTSQGSSL EETIASLKAE VEGFKQERQA VHSEMLLRVQ QLVQDLKKLT CQVATLNNNG EEASTEGTCC PVNWVEHQDS CYWFSHSGMS WAEAEKYCQL KNAHLVVINS REEQNFVQKY LGSAYTWMGL SDPEGAWKWV DGTDYATGFQ NWKPGQPDDW QGHGLGGGED CAHFHPDGRW NDDVCQRPYH WVCEAGLGQT SQESHHHHHH H.

Product Science Overview

Gene and Protein Structure

The CLEC10A gene encodes a protein that is part of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. The human CLEC10A protein consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region.

Expression and Tissue Distribution

CLEC10A is prominently expressed by human peripheral immature dendritic cells, dendritic cells in the skin, alternatively-activated (M2a) macrophages, and to lesser extents by several other types of tissues. The expression pattern suggests its involvement in immune response and cell signaling.

Biological Functions

CLEC10A binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, which are known as Tn-Ag and are expressed in a variety of carcinoma cells. This binding property indicates its role in recognizing and responding to specific carbohydrate structures on pathogens or damaged cells, thereby participating in the regulation of adaptive and innate immune responses.

Recombinant Human CLEC10A

Recombinant human CLEC10A is produced using a mouse myeloma cell line, NS0-derived human CLEC10A/CD301 protein, with an N-terminal 6-His tag. The recombinant protein is used in various research applications, including studying its binding properties and potential therapeutic uses.

Research and Applications

CLEC10A has been studied for its potential role in immunotherapeutic strategies, particularly in targeting ovarian cancer. Its ability to bind specific carbohydrate structures makes it a valuable tool in understanding immune responses and developing targeted therapies.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Product Name

Quantity&Size*

Message

CLEC10A Human

Description

Product Specs

Product Science Overview

Quick Inquiry

Category

Service