CLDN4 Human

Claudin-4 Human Recombinant

Cat. No.

BT185

Source

Escherichia Coli.

Synonyms

Claudin 4, CPE-R, CPE-receptor, Clostridium perfringens enterotoxin receptor 1, WBSCR8, Williams-Beuren syndrome chromosomal region 8 protein, CPETR1.

Appearance

Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

The CLDN4 Protein Human is produced in E. coli, and its molecular weight is 10.63 kDa. The protein containing 93 amino acid residues of the human CLDN4,10 amino acid N-Terminal HisTag residues and 8 additional amino acid PreScission protease cleavage site. The protein is a fusion protein consisting of two extracellular domains of claudin-4 linked together with the PreScission protease LEVLFQGP cleavage site.

Product Specs

Introduction

The CLDN4 gene provides instructions for creating the CLDN4 protein, an integral membrane protein belonging to the claudin family. This protein is crucial for forming tight junction strands, which act as barriers between cells. CLDN4 plays a vital role in the development and functionality of internal organs both before and after birth. Notably, individuals with Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems, often lack the CLDN4 gene.

Description

The CLDN4 Protein Human, a product of E. coli expression, has a molecular weight of 10.63 kDa. It consists of 93 amino acids from the human CLDN4 sequence, a 10 amino acid N-Terminal HisTag, and an 8 amino acid PreScission protease cleavage site. This fusion protein comprises two extracellular domains of claudin-4 connected by the PreScission protease LEVLFQGP cleavage site.

Physical Appearance

White powder, lyophilized and filtered.

Formulation

The product is filtered through a 0.4 μm filter and lyophilized from a 0.5 mg/ml solution in 0.05M Acetate buffer with a pH of 4.

Solubility

To prepare a working stock solution of about 0.5 mg/ml, add 0.1M Acetate buffer (pH 4) to the lyophilized pellet and allow it to dissolve completely. For use at a higher pH, dilute the solution significantly with the appropriate buffer to a concentration of 10 µg/ml. This antigen has limited solubility at higher concentrations. Please note that this product is not sterile. Before using in cell culture, sterilize the product by filtering it through a suitable sterile filter.

Stability

Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freeze-thaw cycles. The reconstituted protein remains stable at 4°C for a short time; no significant changes were observed after two weeks at this temperature.

Purity

SDS-PAGE analysis indicates a purity greater than 90.0%.

Synonyms

Claudin 4, CPE-R, CPE-receptor, Clostridium perfringens enterotoxin receptor 1, WBSCR8, Williams-Beuren syndrome chromosomal region 8 protein, CPETR1.

Source

Escherichia Coli.

Amino Acid Sequence

MKHHHHHHAS MWRVTAFIGS NIVTSQTIWE GLWMNCVVQS TGQMQCKVYD SLLALPQDLQ AAR LEVLFQG PTAHNIIQDF YNPLVASGQK REM MWRVTAFIGS NIVTSQTIWE GLWMNCVVQS TGQMQCKVYD SLLALPQDLQ AAR LEVLFQG PTAHNIIQDF YNPLVASGQK REM.

Product Science Overview

Structure and Function

Claudin-4 is a member of the claudin family, which consists of 27 different proteins. These proteins are integral components of tight junction strands and are involved in the regulation of paracellular transport, which controls the movement of ions and small molecules between cells. Claudin-4, in particular, is known for its role in maintaining the barrier function of epithelial layers in organs such as the intestines and lungs.

Expression and Regulation

Claudin-4 is highly expressed in various epithelial tissues and is particularly abundant in epithelial ovarian carcinomas (EOC) and pancreatic ductal adenocarcinoma. Its expression is regulated by several factors, including epigenetic modifications, inflammatory cytokines, and growth factor signaling. Changes in Claudin-4 expression have been associated with cancer progression and metastasis .

Role in Cancer

Claudin-4 is overexpressed in many epithelial malignancies and is correlated with cancer progression. It helps maintain the tumor microenvironment by forming tight junctions, which act as barriers to the entry of anticancer drugs into tumors. Decreased expression of Claudin-4 is a potential marker of epithelial-mesenchymal transition (EMT), a process that contributes to cancer invasion and metastasis. Non-tight junction Claudin-4 also activates integrin beta 1 and YAP, promoting proliferation, EMT, and stemness .

Therapeutic Potential

Given its significant role in cancer, Claudin-4 has been investigated as a potential molecular target for cancer therapy. Various approaches, such as using anti-Claudin-4 extracellular domain antibodies, gene knockdown, and Clostridium perfringens enterotoxin (CPE), have shown experimental efficacy in targeting Claudin-4. These therapies aim to disrupt the tight junction barrier function and enhance the delivery of anticancer drugs to tumors.

Recombinant Human Claudin-4

Recombinant human Claudin-4 is produced using DNA sequences encoding the full-length protein, which is then expressed in host cells such as HEK293 cells. The recombinant protein is used in various research applications, including studies on tight junction function, cancer progression, and potential therapeutic interventions. It is typically supplied in a sterile buffer and stored under specific conditions to maintain its stability and activity.

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CLDN4 Human

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