CLCF1 Human
Cardiotrophin-Like Cytokine Factor 1 Human Recombinant
Cat. No.
BT30356
Source
Escherichia Coli.
Synonyms
Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3, BSF-3, Novel neurotrophin-1, NNT-1, CLCF1, BSF3, CLC, NNT1, NR6, CISS2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CLCF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (28-225 a.a) and having a molecular mass of 24.6kDa.
CLCF1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CLCF1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Cardiotrophin-like cytokine factor 1 (CLCF1), part of the interleukin 6 cytokine family, signals cells by phosphorylating gp130. This cytokine is comprised of 225 amino acids, including a 27 amino acid signal peptide, resulting in a mature form with a molecular mass of 22kDa. Notably, it shares the highest homology with cardiotrophin-1 and ciliary neurotrophic factor. CLCF1 is actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. Genetic defects in CLCF1 are linked to cold-induced sweating syndrome 2 (CISS2), characterized by excessive sweating after cold exposure and congenital physical abnormalities in the head and spine.
Description
Recombinant human CLCF1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 219 amino acids (28-225 a.a). It has a molecular mass of 24.6kDa. This protein includes a 21 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The CLCF1 protein solution (1mg/ml) is supplied in a buffer containing 20mM sodium citrate (pH 3.5), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3, BSF-3, Novel neurotrophin-1, NNT-1, CLCF1, BSF3, CLC, NNT1, NR6, CISS2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF.
Product Science Overview
Structure and Function
CLCF1 forms a heterodimeric complex with Cytokine Receptor-Like Factor 1 (CRLF1) . This complex competes with Ciliary Neurotrophic Factor (CNTF) for binding to the Ciliary Neurotrophic Factor Receptor (CNTFR) complex, thereby activating the Jak-STAT signaling cascade . The CLCF1-CRLF1 complex can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR .
Biological Roles
- Neuronal Development: CLCF1 is a potent neurotrophic factor that plays a vital role in the survival and differentiation of neuronal cells .
- Immune Regulation: It acts as a B-cell stimulatory agent, enhancing the proliferation and differentiation of B-cells .
- Neuroendocrine Modulation: CLCF1 influences pituitary corticotroph function, thereby modulating neuroendocrine activities .
Clinical Significance
Mutations in the CLCF1 gene are associated with Crisponi/Cold-Induced Sweating Syndrome 2 (CISS2) . This syndrome is characterized by profuse sweating after exposure to cold and congenital physical abnormalities of the head and spine . The CLCF1-CRLF1 complex has also been implicated in various developmental and pathological conditions, highlighting its importance in both health and disease .
Research and Therapeutic Potential
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
