CKS1B Human

CDC28 Protein Kinase Regulatory Subunit 1B Human Recombinant
Cat. No.
BT30733
Source
Escherichia Coli.
Synonyms
CDC28 Protein Kinase Regulatory Subunit 1B, CDC28 Protein Kinase 1B, Cyclin-Dependent Kinases Regulatory Subunit 1, NB4 Apoptosis/Differentiation Related Protein, CDC2-Associated Protein CKS1, Cell Division Control Protein CKS1, CDC28 Protein Kinase 1, PNAS-143, PNAS-16, PNAS-18, CKS-1, ckshs1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CKS1B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 102 amino acids (1-79) and having a molecular mass of 12.0 kDa. CKS1B is fused to a 23 amino acid His-tag at N-terminus.

Product Specs

Introduction
The protein CKS1B is essential for the biological function of cyclin-dependent kinases by binding to their catalytic subunit. Multiple forms of CKS1B mRNA are expressed in HeLa cells throughout the cell cycle, suggesting that the protein it encodes has a specific role. While two transcript variants have been found for this gene, only one appears to code for a protein.
Description
Recombinant human CKS1B, produced in E. coli, is a single, non-glycosylated polypeptide chain with 102 amino acids (residues 1-79) and a molecular mass of 12.0 kDa. A 23 amino acid His-tag is fused to the N-terminus of CKS1B.
Physical Appearance
A clear solution that has been sterile filtered.
Formulation
The CKS1B solution (1mg/ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 150mM NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
CDC28 Protein Kinase Regulatory Subunit 1B, CDC28 Protein Kinase 1B, Cyclin-Dependent Kinases Regulatory Subunit 1, NB4 Apoptosis/Differentiation Related Protein, CDC2-Associated Protein CKS1, Cell Division Control Protein CKS1, CDC28 Protein Kinase 1, PNAS-143, PNAS-16, PNAS-18, CKS-1, ckshs1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSHKQIY YSDKYDDEEF EYRHVMLPKD IAKLVPKTHL MSESEWRNLG VQQSQGWVHY MIHEPEPHIL LFRRPLPKKP KK

Product Science Overview

Introduction

CDC28 Protein Kinase Regulatory Subunit 1B, also known as CKS1B, is a protein-coding gene that plays a crucial role in cell cycle regulation. It is a member of the CKS family and is involved in the modulation of cell cycle function by binding to the catalytic subunit of cyclin-dependent kinases (CDKs). This binding is essential for the biological function of CDKs, which are key regulators of cell cycle progression .

Gene and Protein Information

The CKS1B gene is located on chromosome 1 and is expressed in various tissues throughout the body. The gene produces a protein that is ubiquitously expressed in the nucleus and is involved in several cellular processes, including the regulation of the G1/S transition in the cell cycle . The protein has been found to interact with multiple other proteins, highlighting its importance in cellular function .

Expression and Localization

CKS1B mRNA is expressed in different patterns throughout the cell cycle in HeLa cells, indicating a specialized role for the encoded protein . The protein is localized to the mitochondria, cytosol, nucleoplasm, and vesicles, suggesting its involvement in various cellular compartments and processes .

Biological Function

CKS1B binds to the catalytic subunit of cyclin-dependent kinases and is essential for their biological function. This interaction is crucial for the regulation of the cell cycle, particularly the transition from the G1 phase to the S phase . The protein’s role in cell cycle regulation makes it a key player in maintaining cellular homeostasis and preventing uncontrolled cell proliferation.

Clinical Significance

Mutations or dysregulation of the CKS1B gene have been associated with several diseases, including breast cancer and acute lymphoblastic leukemia . The protein’s involvement in cell cycle regulation and its interaction with CDKs make it a potential target for therapeutic interventions in cancer treatment.

Research and Applications

Human recombinant CKS1B protein is used in various research applications to study its function and interactions with other proteins. It is also utilized in drug discovery and development to identify potential inhibitors that can modulate its activity and provide therapeutic benefits for diseases associated with its dysregulation .

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