CHST3 Human
Sf9, Baculovirus cells.
Carbohydrate sulfotransferase 3, Chondroitin 6-O-sulfotransferase 1, C6ST-1, Chondroitin 6-sulfotransferase, GST-0, CHST3, CHST-3,C6ST1HSD.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
CHST3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 450 amino acids (39-479.a.a) and having a molecular mass of 51.3kDa.
CHST3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Carbohydrate Sulfotransferase 3 (CHST3) is a member of the sulfotransferase 1 family, which consists of 14 enzymes. All members of this family are Golgi-localized type II membrane proteins. These enzymes utilize 3'-phospho-5'-adenylyl sulfate (PAPS) as a sulfate donor to catalyze the transfer of sulfate to the 6th position of the N-acetylgalactosamine (GalNAc) residue of chondroitin. CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. This enzyme is expressed in various tissues including the heart, placenta, skeletal muscle, and pancreas. Additionally, CHST3 plays a role in maintaining naive T-lymphocytes within the spleen.
Produced in Sf9 Baculovirus cells, CHST3 is a single, glycosylated polypeptide chain. It consists of 450 amino acids (39-479.a.a) and has a molecular weight of 51.3kDa. The protein is expressed with a 6 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques.
Sterile filtered, colorless solution.
The CHST3 protein solution is provided at a concentration of 0.25mg/ml. It is formulated in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For longer-term storage, it is recommended to store the product frozen at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. It is important to avoid repeated freeze-thaw cycles to maintain product integrity.
The purity of CHST3 is greater than 90.0% as determined by SDS-PAGE analysis.
CHST3 exhibits a specific activity greater than 1,000 pmol/min/ug. This activity is defined as the amount of enzyme required to transfer sulfate from PAPS to Chondroitin Sulfate per minute at a pH of 7.5 and a temperature of 25°C.
Carbohydrate sulfotransferase 3, Chondroitin 6-O-sulfotransferase 1, C6ST-1, Chondroitin 6-sulfotransferase, GST-0, CHST3, CHST-3,C6ST1HSD.
Sf9, Baculovirus cells.
ADLEKENKII SRVSDKLKQI PQALADANST DPALILAENA SLLSLSELDS AFSQLQSRLR NLSLQLGVEP AMEAAGEEEE EQRKEEEPPR PAVAGPRRHV LLMATTRTGS SFVGEFFNQQ GNIFYLFEPL WHIERTVSFE PGGANAAGSA LVYRDVLKQL FLCDLYVLEH FITPLPEDHL TQFMFRRGSS RSLCEDPVCT PFVKKVFEKY HCKNRRCGPL NVTLAAEACR RKEHMALKAV RIRQLEFLQP LAEDPRLDLR VIQLVRDPRA VLASRMVAFA GKYKTWKKWL DDEGQDGLRE EEVQRLRGNC ESIRLSAELG LRQPAWLRGR YMLVRYEDVA RGPLQKAREM YRFAGIPLTP QVEDWIQKNT QAAHDGSGIY STQKNSSEQF EKWRFSMPFK LAQVVQAACG PAMRLFGYKL ARDAAALTNR SVSLLEERGT FWVTHHHHHH.
Product Science Overview
CHST3 catalyzes the transfer of sulfate groups from the donor molecule 3’-phospho-5’-adenylyl sulfate (PAPS) to the N-acetylgalactosamine (GalNAc) residues of chondroitin. This sulfation process is essential for the formation of chondroitin sulfate, which is a major component of cartilage and other connective tissues . The enzyme can also sulfates the Gal residues of keratan sulfate and sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides, although with lower efficiency .
Chondroitin sulfate, the product of CHST3 activity, is involved in various biological processes, including cell migration, differentiation, and maintenance of the extracellular matrix. It is particularly important in the development and function of cartilage, making CHST3 crucial for skeletal development and maintenance .
Mutations in the CHST3 gene are associated with several genetic disorders, including spondyloepiphyseal dysplasia with congenital joint dislocations and Larsen syndrome. These conditions are characterized by skeletal abnormalities, highlighting the importance of proper CHST3 function in human health .
Recombinant human CHST3 is produced using Chinese Hamster Ovary (CHO) cells. The recombinant protein is typically tagged with a 6-His tag at the N-terminus to facilitate purification. The recombinant CHST3 protein is used in various research applications, including enzyme activity assays and studies on sulfation processes .
