Chitodextrinase

Chitodextrinase from Clostridium botulinum is produced recombinantly in Escherichia coli (E. coli). The recombinant form of this enzyme is fused with a 13 amino acid His tag at the N-terminus, resulting in a single, non-glycosylated polypeptide chain containing 590 amino acids and having a molecular mass of approximately 66.9 kDa . The enzyme is purified using proprietary chromatographic techniques to ensure high purity, typically greater than 95% as determined by SDS-PAGE .

The recombinant Chitodextrinase is typically provided as a sterile, lyophilized (freeze-dried) white powder. It is recommended to reconstitute the lyophilized enzyme in sterile 18MΩ-cm H2O at a concentration of not less than 100 µg/ml, which can then be further diluted into other aqueous solutions . The enzyme is stable at room temperature for up to three weeks when lyophilized, but for long-term storage, it should be kept desiccated below -18°C. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term storage .

Chitodextrinase is an endoenzyme that specifically targets and cleaves soluble chitin oligosaccharides into smaller units, such as di- and trisaccharides . This enzyme is particularly useful in research settings for studying the degradation of chitin and its derivatives. It has applications in various fields, including biotechnology, agriculture, and environmental science, where understanding chitin degradation is essential.

The recombinant Chitodextrinase from Clostridium botulinum is intended for laboratory research use only. It is not suitable for use as a drug, agricultural or pesticidal product, food additive, or household chemical . Proper handling and storage conditions should be followed to maintain the enzyme’s activity and stability.