CHD4 Human

Chromodomain Helicase DNA Binding Protein 4 Human Recombinant
Cat. No.
BT30367
Source
Sf9 insect cells.
Synonyms
Chromodomain Helicase DNA binding protein 4, Mi-2b, Mi2-BETA, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, EC 3.6.4.12, EC 3.6.1.
Appearance
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CHD4 is a full-length cDNA coding for the human Mi-2 beta isoform having a molecular mass of 221,298 Dalton (pH 5.8). CHD4 protein is fused to a hexa-histidine purification tag.

Product Specs

Introduction
The protein CHD4, part of the chromodomain helicase-DNA-binding protein family, plays a crucial role in transcription regulation as a component of the nucleosome remodeling and deacetylase (NuRD) complex. Autoantibodies targeting CHD4 serve as a serological marker for idiopathic inflammatory myopathies (IIM), particularly Mi-2 antibodies, exhibiting high diagnostic specificity (98-100%) and moderate sensitivity (4-18%). Notably, anti-CHD4 antibodies are linked to dermatomyositis, with a frequency of up to 31%, making them valuable for predicting this IIM subset. These antibodies stand out as the only myositis-specific autoantibodies with a clear nuclear target. Furthermore, the prevalence of Mi-2 antibodies is comparable in both children and adults.
Description
This product consists of the full-length cDNA sequence encoding the human Mi-2 beta isoform, resulting in a protein with a molecular mass of 221,298 Dalton at a pH of 5.8. The CHD4 protein is fused to a hexa-histidine purification tag to facilitate purification.
Formulation
CHD4 is supplied in a buffer solution containing 20mM HEPES (pH 8.0) and 500mM NaCl.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Repeated freezing and thawing should be avoided.
Purity
The purity of this product is greater than 95% as determined by SDS-PAGE analysis.
Immunological Functions
This product can be used for: 1. Binding IgG-type human auto-antibodies. 2. As a standard in ELISA tests (e.g., checkerboard analysis of positive/negative sera panels) and immunodot tests with positive/negative sera panels.
Applications
This product can be used for Western blot analysis with myositis sera or a monoclonal anti-hexa-His-tag antibody.
Coating Concentration
The recommended coating concentration for this product is 0.3-0.7 µg/ml, which may vary depending on the ELISA plate type and coating buffer. It is suitable for biotinylation and iodination.
Synonyms
Chromodomain Helicase DNA binding protein 4, Mi-2b, Mi2-BETA, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, EC 3.6.4.12, EC 3.6.1.
Source
Sf9 insect cells.

Product Science Overview

Structure and Function

CHD4 is an ATP-dependent helicase that binds and distorts nucleosomal DNA. It is a central component of the Nucleosome Remodeling and Deacetylase (NuRD) complex, which is involved in the remodeling of chromatin and histone deacetylation . The NuRD complex participates in transcriptional repression and double-strand break repair by homologous recombination .

Role in Epigenetic Regulation

CHD4 is essential for epigenetic transcriptional repression. It localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, promoting transcriptional repression and facilitating DNA repair . This protein is also involved in neurogenesis, highlighting its importance in developmental processes .

Clinical Significance

Mutations in the CHD4 gene are associated with several disorders, including Sifrim-Hitz-Weiss Syndrome and Pallister-Hall Syndrome . Additionally, patients with dermatomyositis often develop antibodies against CHD4, indicating its role in autoimmune responses . Somatic mutations in CHD4 have also been linked to serous endometrial tumors .

Recombinant CHD4

Recombinant CHD4 is a full-length cDNA coding for the human Mi-2 beta protein. It is used in various research applications to study its function and role in chromatin remodeling and transcriptional regulation .

Pathways and Interactions

CHD4 is involved in several key pathways, including RNA Polymerase I Promoter Opening and PIP3 activates AKT signaling . It interacts with multiple components of the NuRD complex, such as MTA1/2, HDAC1, and RbAp46 .

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