CGB3 Human

The CGB3 gene is located on chromosome 19q13.3 and is part of a cluster of six genes arranged in tandem and inverted pairs. These genes are contiguous with the luteinizing hormone beta subunit gene . The CGB3 protein is a heterodimer consisting of a common alpha subunit and a unique beta subunit, which confers biological specificity .

The recombinant form of CGB3 is produced in various expression systems, including Escherichia coli and glycoengineered human cell lines. The recombinant protein typically includes a His-tag for purification purposes and is often used in research and diagnostic applications .

Recombinant CGB3 is produced using advanced biotechnological methods. In Escherichia coli, the protein is expressed as a single, non-glycosylated polypeptide chain containing 168 amino acids with a molecular mass of approximately 17.9 kDa. The protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques .

In glycoengineered human cell lines, the recombinant CGB3 protein is produced with human post-translational modifications, which are crucial for its biological activity. This expression system ensures that the protein is glycosylated, mimicking the natural form of the protein in the human body .

Recombinant CGB3 is widely used in research to study its role in reproductive biology and its potential therapeutic applications. It is also used in diagnostic assays to detect and measure levels of CG in various clinical settings. The protein’s ability to stimulate steroid synthesis in the ovaries makes it a valuable tool in fertility treatments and pregnancy monitoring .

Recombinant CGB3 should be stored at 4°C if used within 2-4 weeks. For longer storage periods, it is recommended to freeze the protein at -20°C with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation. Multiple freeze-thaw cycles should be avoided to maintain the protein’s stability and activity .