CFL1 Antibody

Cofilin-1, Mouse Anti Human
Cat. No.
BT14510
Source
Synonyms
CFL-1, CFL1, Cofilin1, Cofilin-1, Cofilin non-muscle isoform, 18 kDa phosphoprotein, p18.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Cofilin, a family of actin-binding proteins, plays a crucial role in actin filament disassembly. This ubiquitous intracellular protein exhibits pH-dependent activity, binding to and depolymerizing filamentous F-actin while inhibiting the polymerization of monomeric G-actin. Furthermore, cofilin participates in the translocation of the actin-cofilin complex from the cytoplasm to the nucleus.
Formulation
This product is supplied as a 1 mg/ml solution in PBS at a pH of 7.4, supplemented with 0.1% sodium azide.
Storage Procedures
For storage durations of up to one month, maintain the product at 4°C. For extended storage periods, store at -20°C. Minimize freeze-thaw cycles to ensure product integrity.
Stability / Shelf Life
The product remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The CFL1 antibody has undergone rigorous testing through ELISA and Western blot analysis to confirm its specificity and reactivity. However, it is crucial to optimize the reagent titration for each specific application to achieve optimal results. For Western blot analysis, a dilution range of 1:1,000 to 1:2,000 is recommended, with a starting dilution of 1:1,000.
Synonyms
CFL-1, CFL1, Cofilin1, Cofilin-1, Cofilin non-muscle isoform, 18 kDa phosphoprotein, p18.
Purification Method
CFL1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1C1AT.
Immunogen
Anti-human CFL1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CFL1 amino acids 1-166 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Introduction

Cofilin-1, also known as non-muscle cofilin or CFL1, is a member of the actin-depolymerizing factor (ADF)/cofilin family. This family of proteins is essential for the regulation of actin dynamics, which is crucial for various cellular processes such as cytokinesis, endocytosis, and cell motility .

Structure and Function

Cofilin-1 is a widely distributed intracellular protein that binds to actin filaments (F-actin) and monomeric actin (G-actin) in a pH-dependent manner. It plays a pivotal role in actin filament turnover by severing and depolymerizing actin filaments, thus facilitating the rapid reorganization of the actin cytoskeleton . This activity is regulated by phosphorylation at Ser3, which inhibits its actin-binding ability .

Role in Cellular Processes

Cofilin-1 is involved in various cellular processes:

  • Cytokinesis: It helps in the separation of daughter cells during cell division.
  • Endocytosis: It aids in the internalization of cell surface receptors and other molecules.
  • Cell Motility: It is crucial for cell movement by regulating the dynamics of the actin cytoskeleton .
Pathological Implications

Dysregulation of cofilin-1 has been implicated in several neurodegenerative diseases, including Alzheimer’s, Parkinson’s, and amyotrophic lateral sclerosis (ALS). In these conditions, cofilin-1 contributes to the formation of pathological aggregates and synaptic dysfunction . Additionally, cofilin-1 is involved in the pathogenesis of stroke by mediating neurotoxicity and neuronal cell death .

Therapeutic Potential

Given its central role in actin dynamics and its involvement in various diseases, cofilin-1 is a potential therapeutic target. Inhibitors of cofilin-1 and gene therapy approaches are being explored as potential treatments for neurodegenerative diseases and stroke .

Mouse Anti Human Cofilin-1 Antibody

The mouse anti-human cofilin-1 antibody is a polyclonal antibody produced by immunizing mice with a synthetic peptide corresponding to residues surrounding Ser3 of human cofilin-1. This antibody is used in various applications, including Western blotting, to detect endogenous levels of cofilin-1 in human, mouse, rat, and other species .

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Source
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