CFD Human
Human Plasma.
Complement factor D, Adipsin, C3 convertase activator, Properdin factor D, CFD, DF, PFD.
Sterile filtered solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
Human Complement Factor D produced in Human plasma is glycosylated polypeptide chain having a total molecular mass of 24kDa.
Product Specs
Complement Factor D (CFD) is a crucial component of the alternative complement pathway, which is responsible for immune defense. CFD activates factor B in the presence of C3b or C3b-like proteins (e.g., C3, CVF), leading to the formation of the C3 convertase complex (C3b,Bb). This complex converts C3 to its active form (C3b) by cleaving a small peptide (C3a), ultimately contributing to the complement cascade and immune response.
Human Complement Factor D is a glycosylated polypeptide with a molecular weight of 24kDa. It is naturally produced in human plasma.
Sterile, filtered liquid solution.
The CFD protein solution is formulated in a buffer containing 10mM Sodium phosphate and 145mM NaCl, at a pH of 7.3.
For short-term storage (2-4 weeks), Human CFD can be stored at 4°C. For extended storage, freeze below -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freezing and thawing.
High purity exceeding 95.0%, as determined by SDS-PAGE analysis.
The plasma used in the production process undergoes rigorous testing to ensure it is free from HIV-1, HIV-2, HCV, and HBSAG antibodies.
Complement factor D, Adipsin, C3 convertase activator, Properdin factor D, CFD, DF, PFD.
Human Plasma.
Product Science Overview
Complement Factor D is a serine protease, a type of enzyme that cleaves peptide bonds in proteins. It is produced by adipocytes (fat cells) and secreted into the bloodstream . Its primary role is to cleave Factor B when it is bound to C3b, a fragment of the complement protein C3 . This cleavage is essential for the formation of the C3 convertase enzyme complex, which amplifies the complement activation process .
The alternative complement pathway, unlike the classical and lectin pathways, does not require antibodies for activation. Instead, it is continuously active at a low level and can rapidly amplify the immune response upon detection of pathogens .
Complement Factor D plays a significant role in various physiological and pathological processes:
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Infection and Immunity: It is vital for the effective clearance of pathogens. Deficiencies in Complement Factor D can lead to increased susceptibility to infections, particularly by Neisseria species .
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Autoimmune Diseases: Dysregulation of Complement Factor D activity is linked to autoimmune diseases. Excessive activation of the complement system can contribute to tissue damage and inflammation .
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Sepsis: Research has shown that higher levels of Complement Factor D are associated with increased mortality in sepsis patients. This is due to its role in platelet activation and coagulation, which are critical factors in the progression of sepsis .
Given its central role in the alternative complement pathway, Complement Factor D is a strategic target for therapeutic intervention. Inhibitors of Complement Factor D are being explored as potential treatments for diseases characterized by excessive complement activation, such as certain autoimmune and inflammatory conditions .
