CDKN3 Human

Cyclin-Dependent Kinase Inhibitor 3 Human Recombinant
Cat. No.
BT1745
Source
Escherichia Coli.
Synonyms
Cyclin-dependent kinase inhibitor 3, CDK2-associated dual-specificity phosphatase, Cyclin-dependent kinase interactor 1, Cyclin-dependent kinase-interacting protein 2, Kinase-associated phosphatase, KAP, CDI1, CIP2, KAP1, FLJ25787, MGC70625, CDKN3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

CDKN3 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 232 amino acids (1-212 a.a.) and having a molecular mass of 25.9kDa. The CDKN3 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cyclin-dependent kinase inhibitor 3 (CDKN3) is a member of the dual specificity protein phosphatase family. It acts as a cyclin-dependent kinase inhibitor by interacting with and dephosphorylating CDK2 kinase, thereby preventing its activation. CDKN3 plays a crucial role in cell cycle regulation. Alterations in CDKN3, such as deletions, mutations, or overexpression, have been observed in various types of cancers.
Description
Recombinant human CDKN3, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 232 amino acids, with a 20 amino acid His tag at the N-terminus (1-212 a.a. of CDKN3), and has a molecular weight of 25.9 kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CDKN3 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, 40% glycerol, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, it is recommended to store the product frozen at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advised. Avoid repeated freeze-thaw cycles.
Purity
The purity of CDKN3 is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Cyclin-dependent kinase inhibitor 3, CDK2-associated dual-specificity phosphatase, Cyclin-dependent kinase interactor 1, Cyclin-dependent kinase-interacting protein 2, Kinase-associated phosphatase, KAP, CDI1, CIP2, KAP1, FLJ25787, MGC70625, CDKN3.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MKPPSSIQTS EFDSSDEEPI EDEQTPIHIS WLSLSRVNCS QFLGLCALPG CKFKDVRRNV QKDTEELKSC GIQDIFVFCT RGELSKYRVP NLLDLYQQCG IITHHHPIAD GGTPDIASCC EIMEELTTCL KNYRKTLIHC YGGLGRSCLV AACLLLYLSD TISPEQAIDS LRDLRGSGAI QTIKQYNYLH EFRDKLAAHL SSRDSQSRSV SR.

Product Science Overview

Introduction

Cyclin-Dependent Kinase Inhibitor 3 (CDKN3), also known as KAP (Kinase-Associated Phosphatase), is a protein encoded by the CDKN3 gene in humans. This protein plays a crucial role in cell cycle regulation by interacting with and dephosphorylating cyclin-dependent kinases (CDKs), particularly CDK2 .

Gene and Protein Structure

The CDKN3 gene is located on chromosome 14 and encodes a dual specificity protein phosphatase. This means that CDKN3 can remove phosphate groups from both tyrosine and serine/threonine residues on its substrates . The protein has several aliases, including CIP2, CDI1, and KAP1 .

Function and Mechanism

CDKN3 is primarily involved in the regulation of the cell cycle. It dephosphorylates CDK2 at the threonine-160 residue, which is essential for CDK2 activation . By doing so, CDKN3 prevents the activation of CDK2, thereby inhibiting cell cycle progression from the G1 to the S phase . This regulatory mechanism is vital for maintaining proper cell division and preventing uncontrolled cell proliferation.

Clinical Significance

CDKN3 has been implicated in various cancers, including hepatocellular carcinoma, breast cancer, and prostate cancer . The gene can be deleted, mutated, or overexpressed in these cancers, leading to dysregulation of the cell cycle and contributing to tumorigenesis . Additionally, CDKN3 has been shown to interact with other CDKs, such as CDK3 and CDC2, further highlighting its role in cell cycle control .

Research and Applications

Recombinant human CDKN3 is used in research to study its function and role in cancer. By understanding how CDKN3 interacts with CDKs and regulates the cell cycle, researchers can develop targeted therapies for cancers where CDKN3 is dysregulated . Various antibodies and assays are available for detecting and studying CDKN3 in laboratory settings .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

CDK5 Human
Cyclin-dependent Kinase 5 Human Recombinant
Cat. No.
BT1361
Source
Escherichia Coli.
CDKN1B Human
Cyclin-Dependent Kinase Inhibitor 1B Human Recombinant
Cat. No.
BT1598
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.