CDK5RAP3 Human

CDK5 Regulatory Subunit Associated Protein 3 Human Recombinant
Cat. No.
BT29783
Source
Escherichia Coli.
Synonyms
CDK5 Regulatory Subunit Associated Protein 3, LXXLL/Leucine-Zipper-Containing ARF-Binding Protein, LXXLL/Leucine-Zipper-Containing ARFbinding Protein, Ischemic Heart CDK5 Activator-Binding Protein C53, IC53, LZAP, CDK5 Regulatory Subunit Associated Protein IC53-2, CDK5 Regulatory Subunit-Associated Protein 3, CDK5 Activator-Binding Protein C53, Protein HSF-27, OK/SW-Cl.114, HSF-27, MST016, PP1553, C53, CDK5RAP3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CDK5RAP3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 526 amino acids (1-506 a.a) and having a molecular mass of 59kDa.
CDK5RAP3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CDK5 Regulatory Subunit Associated Protein 3 (CDK5RAP3) plays a role in regulating CDK5 activity through its interaction with CDK5R. CDK5RAP3 binds to p25NCK5A, a component of the neuronal CDC2-like kinase involved in neuronal differentiation. This kinase consists of a catalytic subunit (CDK5) and an activating subunit (p25NCK5A), suggesting CDK5RAP3's involvement in neuronal differentiation.
Description
Recombinant CDK5RAP3, expressed in E. coli, is a non-glycosylated polypeptide chain with 526 amino acids (1-506 a.a) and a molecular weight of 59kDa. This protein lacks glycosylation and has a His-tag fused at the N-terminus (20 amino acids). Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The CDK5RAP3 protein solution (0.5mg/ml) is supplied in a buffer containing phosphate-buffered saline (pH 7.4), 20% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
SDS-PAGE analysis indicates a purity greater than 90.0%.
Synonyms
CDK5 Regulatory Subunit Associated Protein 3, LXXLL/Leucine-Zipper-Containing ARF-Binding Protein, LXXLL/Leucine-Zipper-Containing ARFbinding Protein, Ischemic Heart CDK5 Activator-Binding Protein C53, IC53, LZAP, CDK5 Regulatory Subunit Associated Protein IC53-2, CDK5 Regulatory Subunit-Associated Protein 3, CDK5 Activator-Binding Protein C53, Protein HSF-27, OK/SW-Cl.114, HSF-27, MST016, PP1553, C53, CDK5RAP3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MEDHQHVPID IQTSKLLDWL VDRRHCSLKW QSLVLTIREK INAAIQDMPE SEEIAQLLSG SYIHYFHCLR ILDLLKGTEA STKNIFGRYS SQRMKDWQEI IALYEKDNTY LVELSSLLVR NVNYEIPSLK KQIAKCQQLQ QEYSRKEEEC QAGAAEMREQ FYHSCKQYGI TGENVRGELL ALVKDLPSQL AEIGAAAQQS LGEAIDVYQA SVGFVCESPT EQVLPMLRFV QKRGNSTVYE WRTGTEPSVV ERPHLEELPE QVAEDAIDWG DFGVEAVSEG TDSGISAEAA GIDWGIFPES DSKDPGGDGI DWGDDAVALQ ITVLEAGTQA PEGVARGPDA LTLLEYTETR NQFLDELMEL EIFLAQRAVE LSEEADVLSV SQFQLAPAIL QGQTKEKMVT MVSVLEDLIG KLTSLQLQHL FMILASPRYV DRVTEFLQQK LKQSQLLALK KELMVQKQQE ALEEQAALEP KLDLLLEKTK ELQKLIEADI SKRYSGRPVN LMGTSL.

Product Science Overview

Gene and Protein Structure

The CDK5RAP3 gene is located on chromosome 17q21.32 in humans . It encodes a protein that consists of 526 amino acids and has a molecular mass of approximately 59 kDa . The protein is produced in E. coli as a single, non-glycosylated polypeptide chain and is fused to a 20 amino acid His-tag at the N-terminus for purification purposes .

Function and Mechanism

CDK5RAP3 is known to interact with the cyclin-dependent kinase 5 (CDK5) regulatory subunit, CDK5R1, and acts as a potential regulator of CDK5 activity . CDK5 is a neuronal CDC2-like kinase involved in the regulation of neuronal differentiation. It is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A .

The protein has been reported to function in several signaling pathways, including those governing transcriptional regulation and cell cycle progression . It negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation . Additionally, CDK5RAP3 is involved in the mitotic G2/M transition checkpoint and the mitotic G2 DNA damage checkpoint .

Role in Disease

CDK5RAP3 has been implicated in tumorigenesis and metastasis . It is considered a probable tumor suppressor, initially identified as a CDK5R1 interactor controlling cell proliferation . Through its interaction with CDKN2A/ARF and MDM2, CDK5RAP3 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization, and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation .

Recombinant Protein Production

The human recombinant CDK5RAP3 protein is produced in E. coli and purified using proprietary chromatographic techniques . This recombinant protein is used in various research applications to study its function and role in different cellular processes.

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