CDK5 Human, Sf9
CDK5, Cyclin Dependent Kinase 5, Serine/Threonine-Protein Kinase PSSALRE, Tau Protein Kinase II Catalytic Subunit, Cell Division Protein Kinase 5, TPKII Catalytic Subunit, Cyclin-Dependent-Like Kinase 5, Protein Kinase CDK5 Splicing, EC 2.7.11.22, EC 2.7.11.1, EC 2.7.11, PSSALRE, CDKN5, LIS7.
Greater than 90.0% as determined by SDS-PAGE.
Description
CDK5 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 298 amino acids (1-292a.a.) and having a molecular mass of 34.1kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
CDK5 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CDK5, Cyclin Dependent Kinase 5, Serine/Threonine-Protein Kinase PSSALRE, Tau Protein Kinase II Catalytic Subunit, Cell Division Protein Kinase 5, TPKII Catalytic Subunit, Cyclin-Dependent-Like Kinase 5, Protein Kinase CDK5 Splicing, EC 2.7.11.22, EC 2.7.11.1, EC 2.7.11, PSSALRE, CDKN5, LIS7.
MQKYEKLEKI GEGTYGTVFK AKNRETHEIV ALKRVRLDDD DEGVPSSALR EICLLKELKH KNIVRLHDVL HSDKKLTLVF EFCDQDLKKY FDSCNGDLDP EIVKSFLFQL LKGLGFCHSR NVLHRDLKPQ NLLINRNGEL KLADFGLARA FGIPVRCYSA EVVTLWYRPP DVLFGAKLYS TSIDMWSAGC IFAELANAGR PLFPGNDVDD QLKRIFRLLG TPTEEQWPSM TKLPDYKPYP MYPATTSLVN VVPKLNATGR DLLQNLLKCN PVQRISAEEA LQHPYFSDFC PPHHHHHH
Product Science Overview
Cyclin-Dependent Kinase 5 (CDK5) is a proline-directed serine/threonine protein kinase that belongs to the cyclin-dependent kinase family. Unlike other members of this family, CDK5 does not directly control cell cycle regulation. Instead, it plays a crucial role in the nervous system, particularly in the development and function of neurons .
CDK5 was first discovered due to its sequence homology to the human cell division cycle protein 2 (Cdc2, also known as CDK1), a regulator of cell cycle progression . Despite its similarity to other cyclin-dependent kinases, CDK5’s activity is primarily observed in post-mitotic neurons, where it is essential for neuronal cell cycle arrest and differentiation .
CDK5 requires association with a regulatory subunit to become active. Unlike other CDKs that associate with cyclins, CDK5 is activated by binding to non-cyclin proteins such as p35 and p39 . These regulatory subunits are primarily expressed in the brain and are crucial for the kinase activity of CDK5 .
CDK5 is involved in various cellular processes in neurons, including:
- Synaptic Plasticity: CDK5 regulates synaptic plasticity, which is essential for learning and memory .
- Neuronal Migration: It plays a role in the migration of neurons during brain development .
- Cytoskeletal Organization: CDK5 phosphorylates proteins required for cytoskeletal organization, endocytosis, and exocytosis .
- Apoptosis: It may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry .
Recombinant CDK5 is produced using the Sf9 insect cell expression system. This system is widely used for the production of recombinant proteins due to its ability to perform post-translational modifications similar to those in mammalian cells . The recombinant CDK5 produced in Sf9 cells retains its kinase activity and can be used for various research applications, including studying its role in neuronal functions and neurodegenerative diseases .
