CDK4 Antibody
Description
Product Specs
The antibody is supplied at a concentration of 1 milligram per milliliter (mg/ml) and is dissolved in a solution consisting of phosphate-buffered saline (PBS) with a pH of 7.4, 0.02% sodium azide (as a preservative), and 10% glycerol (as a stabilizing agent).
Product Science Overview
Cyclin-Dependent Kinase 4 (CDK4) is a crucial enzyme that plays a significant role in the regulation of the cell cycle. It is a member of the Ser/Thr protein kinase family and is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2 . CDK4, in association with D-type cyclins, is essential for the progression of cells through the G1 phase of the cell cycle .
CDK4 forms a complex with D-type cyclins (Cyclin D1, D2, and D3), which acts as a kinase by phosphorylating the retinoblastoma (Rb) protein . This phosphorylation event releases the E2F transcription factor, which is necessary for the transition from the G1 phase to the S phase of the cell cycle . The activity of CDK4 is tightly regulated by CDK inhibitors, such as p16^INK4a, which bind to CDK4 and prevent its activation .
Abnormal levels or activation states of CDK4 are closely associated with tumorigenesis and the progression of various cancers . Amplification or rearrangements of the CDK4-encoding gene can lead to overexpression of the CDK4 protein, which is commonly observed in many types of solid tumors and hematologic malignancies . Additionally, the inactivation of CDK4 inhibitor proteins, such as p16^INK4a, is frequently seen in cancers, leading to excessive activation of CDK4 kinase and uncontrolled cell proliferation .
Mouse anti-human CDK4 antibodies are monoclonal antibodies developed to specifically target and bind to human CDK4. These antibodies are valuable tools in research and clinical diagnostics for detecting CDK4 expression in various tissues and cell types. They are used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to study CDK4’s role in cell cycle regulation and its involvement in cancer .
The development of mouse anti-human CDK4 antibodies has significant implications for cancer research and therapy. These antibodies can be used to detect CDK4 expression in tumor cells, aiding in the diagnosis and prognosis of cancers . Furthermore, they can serve as potential therapeutic agents by targeting CDK4 in cancer cells, thereby inhibiting their proliferation and inducing cell cycle arrest .
