CDK2 Human

Cyclin-Dependent Kinase 2 Human Recombinant
Cat. No.
BT752
Source
Escherichia Coli.
Synonyms
Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2, p33(CDK2).
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CDK2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 306 amino acids (1-298 a.a.) and having a molecular mass of 35kDa.
CDK2 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cyclin-dependent kinase 2 (CDK2) is a member of the Ser/Thr protein kinase family and shares significant similarity with the gene products of cdc28 in S. cerevisiae and cdc2 in S. pombe. As a catalytic subunit of the cyclin-dependent protein kinase complex, CDK2 plays a crucial role in regulating the cell cycle's G1-S phase transition, with its activity primarily restricted to this phase. Its activity is modulated by regulatory subunits within the complex, including cyclins A and E, as well as CDK inhibitors p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Moreover, CDK2 activity is subject to regulation through protein phosphorylation.
Description
Recombinant human CDK2, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 306 amino acids (residues 1-298) with a molecular weight of 35 kDa. It comprises the full CDK2 protein sequence with an 8-amino acid His-tag fused at the C-terminus to facilitate purification via proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CDK2 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution is formulated with 20mM Tris-HCl buffer at pH 8.0, 20% glycerol, and 1mM DTT to ensure protein stability and prevent degradation.
Stability
For short-term storage (2-4 weeks), the CDK2 protein solution should be kept at a refrigerated temperature of 4°C. For extended storage, it is recommended to store the solution in a frozen state at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA to a final concentration of 0.1%. It is crucial to minimize repeated freeze-thaw cycles to maintain the protein's integrity and activity.
Purity
The purity of the CDK2 protein is determined to be greater than 95% through SDS-PAGE analysis, indicating a high level of purity.
Synonyms
Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2, p33(CDK2).
Source
Escherichia Coli.
Amino Acid Sequence
MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRLLE HHHHHH.

Product Science Overview

Introduction

Cyclin-Dependent Kinase 2 (CDK2) is a crucial enzyme in the regulation of the cell cycle. It belongs to the family of serine/threonine protein kinases and plays a pivotal role in the transition from the G1 phase to the S phase of the cell cycle, where cells prepare for DNA replication and mitosis .

Structure and Function

CDK2 is highly similar to the gene products of Saccharomyces cerevisiae cdc28 and Schizosaccharomyces pombe cdc2, also known as CDK1 in humans . It acts as a catalytic subunit of the cyclin-dependent kinase complex, whose activity is tightly regulated by its association with cyclins, such as cyclin E and cyclin A . Cyclin E binds to CDK2 during the G1 phase, facilitating the transition to the S phase, while cyclin A binds to CDK2 to ensure progression through the S phase .

Regulation

The activity of CDK2 is regulated by phosphorylation. Inhibitory phosphorylation occurs on threonine 14 and tyrosine 15, while activation requires phosphorylation on threonine 160 . This precise regulation ensures that CDK2 activity is restricted to specific phases of the cell cycle, preventing uncontrolled cell division.

Role in Cell Cycle and Meiosis

CDK2 is essential for the proper progression of the cell cycle. It has been shown to be indispensable for the G1-S phase transition, although recent studies suggest that cells lacking CDK2 can still progress through the cell cycle, albeit with a lengthened G1 phase . CDK2 is also crucial for meiosis, as its deletion in mice leads to reproductive sterility due to the arrest of germ cells at the prophase of meiosis .

Recombinant CDK2

Recombinant human CDK2 is produced using genetic engineering techniques, where the CDK2 gene is cloned and expressed in suitable host cells, such as Escherichia coli or yeast. The recombinant protein is then purified for use in various research applications, including studies on cell cycle regulation, cancer research, and drug development .

Applications in Research

CDK2 is a target for cancer research due to its role in cell cycle regulation. Inhibitors of CDK2 are being investigated as potential therapeutic agents for cancer treatment. Additionally, recombinant CDK2 is used in biochemical assays to study its interactions with cyclins and other regulatory proteins, as well as to identify potential substrates and inhibitors .

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