CDK1 Human, Sf9
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Description
Product Specs
Product Science Overview
Cyclin-Dependent Kinase 1 (CDK1), also known as CDC2, is a crucial enzyme in the regulation of the cell cycle. It plays a pivotal role in the transition from the G2 phase to the M phase, facilitating the onset of mitosis. CDK1 is a serine/threonine kinase that forms a complex with cyclins, particularly Cyclin B, to exert its function. The human recombinant form of CDK1, expressed in Sf9 cells, is widely used in research to study cell cycle regulation and its implications in various diseases, including cancer.
The recombinant expression of CDK1 in Sf9 cells, a type of insect cell line derived from the fall armyworm (Spodoptera frugiperda), is a common method for producing high yields of active protein. The baculovirus expression system used in Sf9 cells allows for post-translational modifications that are essential for the proper function of CDK1. This system is advantageous due to its ability to produce large quantities of protein with high purity and activity .
CDK1 is a 34 kDa protein that requires binding to a regulatory cyclin subunit, such as Cyclin B, to become fully active. The CDK1/Cyclin B complex is essential for the phosphorylation of various substrates that drive the cell cycle forward. This complex is tightly regulated by phosphorylation and dephosphorylation events, ensuring precise control over cell division .
CDK1, in association with Cyclin B, is responsible for initiating several key processes during mitosis, including chromosome condensation, nuclear envelope breakdown, and spindle formation. The activity of CDK1 is regulated by the phosphorylation of a threonine residue in its activation loop by CDK-activating kinase (CAK). Additionally, inhibitory phosphorylation sites must be dephosphorylated for CDK1 to become active .
The human recombinant form of CDK1 expressed in Sf9 cells is extensively used in biochemical and structural studies to understand its role in cell cycle regulation. Researchers utilize this recombinant protein to investigate the mechanisms of CDK1 activation, substrate specificity, and its interactions with other cell cycle regulators. These studies are crucial for developing targeted therapies for diseases characterized by dysregulated cell division, such as cancer .
