CDK16 Human

Cyclin-dependent kinase 16 Human Recombinant
Cat. No.
BT641
Source
Escherichia Coli.
Synonyms
Cyclin-Dependent Kinase 16, PCTK1, Serine/Threonine-Protein Kinase,  Serine/Threonine-Protein Kinase PCTAIRE-1, Cell Division Protein Kinase 16, PCTAIRE Protein Kinase 1, PCTAIRE, PCTGAIRE, EC 2.7.11.22, EC 2.7.11, PCTAIRE-Motif Protein Kinase 1, PCTAIRE1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CDK16 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 362 amino acids (158-496aa) and having a molecular mass of 41.1kDa.
CDK16 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CDK16, a member of the cyclin-dependent kinase (CDK) family, plays a crucial role in cell cycle regulation. This protein kinase features a central kinase domain flanked by distinct amino- and carboxy-terminal regions. Primarily found in the mammalian brain, CDK16 interacts with various proteins and participates in multiple signal transduction pathways.
Description
Recombinant human CDK16, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 362 amino acids (residues 158-496). This protein, with a molecular weight of 41.1 kDa, is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The CDK16 protein is supplied as a 1 mg/ml solution in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the CDK16 protein is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
Cyclin-Dependent Kinase 16, PCTK1, Serine/Threonine-Protein Kinase,  Serine/Threonine-Protein Kinase PCTAIRE-1, Cell Division Protein Kinase 16, PCTAIRE Protein Kinase 1, PCTAIRE, PCTGAIRE, EC 2.7.11.22, EC 2.7.11, PCTAIRE-Motif Protein Kinase 1, PCTAIRE1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSGFGKLET YIKLDKLGEG TYATVYKGKS KLTDNLVALK EIRLEHEEGA PCTAIREVSL LKDLKHANIV TLHDIIHTEK SLTLVFEYLD KDLKQYLDDC GNIINMHNVK LFLFQLLRGL AYCHRQKVLH RDLKPQNLLI NERGELKLAD FGLARAKSIP TKTYSNEVVT LWYRPPDILL GSTDYSTQID MWGVGCIFYE MATGRPLFPG STVEEQLHFI FRILGTPTEE TWPGILSNEE FKTYNYPKYR AEALLSHAPR LDSDGADLLT KLLQFEGRNR ISAEDAMKHP FFLSLGERIH KLPDTTSIFA LKEIQLQKEA SLRSSSMPDS GRPAFRVVDT EF

Product Science Overview

Introduction

Cyclin-dependent kinase 16 (CDK16), also known as PCTAIRE1, is a member of the cyclin-dependent kinase (CDK) family, which plays a crucial role in regulating various cellular processes. Unlike typical CDKs that are primarily involved in cell cycle regulation, CDK16 is classified as an atypical CDK due to its unique structure and functions. CDK16 is predominantly expressed in terminally differentiated cells, such as neurons and testis, and is involved in processes like exocytosis, vesicle-mediated transport, and signal transduction .

Structure and Function

CDK16 belongs to the PCTAIRE family, which includes CDK16, CDK17, and CDK18. These kinases are characterized by a highly conserved kinase domain that is closely related to those of cell cycle-regulating CDKs . CDK16’s activity is dependent on its interaction with Cyclin Y (CCNY), which regulates its kinase activity. This interaction is essential for CDK16’s role in various cellular processes, including the regulation of insulin secretion, spermatogenesis, and neuron differentiation .

Biological Significance

CDK16 has been implicated in several critical biological processes. It plays a role in the regulation of insulin secretion in response to changes in blood glucose levels, which is vital for maintaining glucose homeostasis . Additionally, CDK16 is required for normal spermatogenesis, highlighting its importance in reproductive biology . In neurons, CDK16 regulates differentiation and dendrite development, contributing to proper neuronal function .

Clinical Relevance

Recent studies have shown that CDK16 is highly expressed in certain types of cancer, particularly triple-negative breast cancer (TNBC). Elevated CDK16 expression is correlated with poor outcomes in breast cancer patients, making it a potential therapeutic target . CDK16 promotes the proliferation and migration of TNBC cells by phosphorylating protein regulator of cytokinesis 1 (PRC1), which is involved in spindle formation during mitosis . Both genetic knockdown and pharmacological inhibition of CDK16 have been shown to significantly suppress tumor progression in TNBC .

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