CDCP1 Human

CUB Domain Containing Protein 1 Human Recombinant
Cat. No.
BT29710
Source
Sf9, Baculovirus cells.
Synonyms
CUB Domain Containing Protein 1, Subtractive Immunization M Plus HEp3-Associated 135 KDa Protein, Transmembrane And Associated With Src Kinases, Membrane Glycoprotein Gp140, SIMA135, TRASK, CD318 Antigen, CD318, CUB domain-containing protein 1, Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, Transmembrane and associated with src kinases.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CDCP1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 646 amino acids (30-667a.a.) and having a molecular mass of 72.8kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). CDCP1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
CUB Domain Containing Protein 1, also known as CDCP1, is a transmembrane protein that contains three extracellular CUB domains. This protein plays a role in cell adhesion and cell-matrix association. Additionally, CDCP1 participates in regulating anchorage versus migration and proliferation versus differentiation through phosphorylation. It serves as a novel marker for leukemia diagnosis and immature hematopoietic stem cell subsets. The extracellular region of human CDCP1 shares amino acid identity sequence with its mouse counterpart.
Description
CDCP1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 646 amino acids (30-667a.a.). With a molecular mass of 72.8 kDa, it appears between 70-100 kDa on SDS-PAGE. This protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless, and filtered solution.
Formulation
The CDCP1 protein solution (0.25 mg/ml) is prepared in Phosphate Buffered Saline (pH 7.4) and contains 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
CUB Domain Containing Protein 1, Subtractive Immunization M Plus HEp3-Associated 135 KDa Protein, Transmembrane And Associated With Src Kinases, Membrane Glycoprotein Gp140, SIMA135, TRASK, CD318 Antigen, CD318, CUB domain-containing protein 1, Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, Transmembrane and associated with src kinases.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
FEIALPRESN ITVLIKLGTP TLLAKPCYIV ISKRHITMLS IKSGERIVFT FSCQSPENHF VIEIQKNIDC MSGPCPFGEV QLQPSTSLLP TLNRTFIWDV KAHKSIGLEL QFSIPRLRQI GPGESCPDGV THSISGRIDA TVVRIGTFCS NGTVSRIKMQ EGVKMALHLP WFHPRNVSGF SIANRSSIKR LCIIESVFEG EGSATLMSAN YPEGFPEDEL MTWQFVVPAH LRASVSFLNF NLSNCERKEE RVEYYIPGST TNPEVFKLED KQPGNMAGNF NLSLQGCDQD AQSPGILRLQ FQVLVQHPQN ESNKIYVVDL SNERAMSLTI EPRPVKQSRK FVPGCFVCLE SRTCSSNLTL TSGSKHKISF LCDDLTRLWM NVEKTISCTD HRYCQRKSYS LQVPSDILHL PVELHDFSWK LLVPKDRLSL VLVPAQKLQQ HTHEKPCNTS FSYLVASAIP SQDLYFGSFC PGGSIKQIQV KQNISVTLRT FAPSFQQEAS RQGLTVSFIP YFKEEGVFTV TPDTKSKVYL RTPNWDRGLP SLTSVSWNIS VPRDQVACLT FFKERSGVVC QTGRAFMIIQ EQRTRAEEIF SLDEDVLPKP SFHHHSFWVN ISNCSPTSGK QLDLLFSVTL TPRTVDLTLE HHHHHH.

Product Science Overview

Introduction

CUB Domain Containing Protein 1 (CDCP1), also known as CD318, SIMA135, and TRASK, is a type I transmembrane glycoprotein. It is characterized by the presence of CUB domains, which are involved in protein-protein interactions. CDCP1 is predominantly located on the cell surface and plays a crucial role in various cellular processes, including cell adhesion, migration, and survival.

Structure and Function

CDCP1 is composed of three extracellular CUB domains, a single transmembrane domain, and a short cytoplasmic tail. The CUB domains facilitate interactions with other proteins, while the cytoplasmic tail is involved in intracellular signaling. CDCP1 is known to interact with several key signaling molecules, including SRC family kinases and protein kinase C delta (PKCδ), which are involved in oncogenic signaling pathways .

Role in Cancer

CDCP1 is upregulated in a variety of malignancies, including cancers of the breast, lung, colorectum, ovary, kidney, liver, pancreas, and hematopoietic system . Elevated levels of CDCP1 are associated with progressive disease and poorer survival outcomes. It is implicated in several oncogenic processes, such as:

  • Cell Survival and Growth: CDCP1 promotes cancer cell survival and growth by activating signaling pathways like SRC/PKCδ, PI3K/AKT, and RAS/ERK .
  • Metastasis: CDCP1 enhances the metastatic potential of cancer cells by facilitating cell migration and invasion .
  • Treatment Resistance: CDCP1 contributes to resistance against cytotoxic chemotherapy and targeted therapies, making it a challenging target in cancer treatment .
Therapeutic Potential

Given its significant role in cancer progression, CDCP1 has emerged as a potential biomarker and therapeutic target. Researchers are exploring various strategies to target CDCP1 for cancer diagnosis and treatment. For instance, radioligand therapy (RLT) targeting CDCP1 has shown promise in treating metastatic castration-resistant prostate cancer (mCRPC), including subsets with low prostate-specific membrane antigen (PSMA) expression .

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