CDA Human

Cytidine Deaminase Human Recombinant

Cat. No.

BT4882

Source

Escherichia Coli.

Synonyms

Cytidine deaminase, Cytidine aminohydrolase, CDA, CDD.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

CDA Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids (1-146 a.a.) and having a molecular mass of 18.3kDa. The CDA is purified by proprietary chromatographic techniques.

Product Specs

Introduction

Cytidine deaminase (CDA) plays a crucial role in nucleotide metabolism by breaking down cytidine and deoxycytidine into uridine and deoxyuridine, respectively. This enzyme is essential for regulating the balance of pyrimidine nucleotides within cells. CDA is also known to inactivate certain chemotherapy drugs like Ara-C and 5-azacytidine, which limits their effectiveness. CDA typically exists as a tetramer (a complex of four identical units) and is primarily found in granulocytes, a type of white blood cell. Genetic mutations affecting the CDA gene can lead to reduced sensitivity to cytosine arabinoside, a drug commonly used in treating childhood leukemia.

Description

This product is a recombinant human CDA protein engineered with a 20 amino acid His tag at its N-terminus. It is produced in E. coli and purified to a single, non-glycosylated polypeptide chain of 166 amino acids (specifically amino acids 1-146). With a molecular weight of 18.3kDa, this protein is provided in a solution at a concentration of 0.5mg/ml and has been purified using proprietary chromatographic techniques.

Physical Appearance

A clear and colorless solution that has been sterilized by filtration.

Formulation

The CDA protein is supplied in a solution containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 2mM EDTA, 100mM NaCl, and 40% glycerol.

Stability

For optimal storage, keep refrigerated at 4°C. If using within 2-4 weeks, the product can be stored at 4°C. For long-term storage exceeding this period, it is recommended to freeze at -20°C. To further enhance stability during prolonged storage, consider adding a carrier protein (either HSA or BSA) at a concentration of 0.1%. Minimize repeated freeze-thaw cycles to preserve protein integrity.

Purity

The purity of this protein is greater than 90%, as determined by SDS-PAGE analysis.

Biological Activity

The specific activity of this enzyme is greater than 10,000 pmol/min/µg. This is measured as the amount of enzyme required to deaminate 1.0 picomole of cytidine per minute at a pH of 7.5 and a temperature of 25°C.

Synonyms

Cytidine deaminase, Cytidine aminohydrolase, CDA, CDD.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ.

Product Science Overview

Introduction

Cytidine deaminase (CDA) is an enzyme encoded by the CDA gene in humans. This enzyme plays a crucial role in the pyrimidine salvage pathway, which is essential for maintaining the cellular pyrimidine pool. The enzyme catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively.

Structure and Function

Cytidine deaminase forms a homotetramer, meaning it consists of four identical subunits. This structure is vital for its function, as it allows the enzyme to efficiently catalyze the deamination process. The enzyme’s activity is dependent on zinc ions, which are required for its catalytic function.

Biological Role

Cytidine deaminase is involved in the pyrimidine salvage pathway, which recycles pyrimidine nucleosides for nucleotide synthesis. This pathway is crucial for maintaining the balance of nucleotides within the cell, which is essential for DNA and RNA synthesis. The enzyme’s activity ensures that cytidine and deoxycytidine are converted to uridine and deoxyuridine, preventing the accumulation of cytidine and maintaining the cellular nucleotide pool.

Clinical Significance

Mutations in the CDA gene can lead to decreased sensitivity to cytosine nucleoside analogs, such as cytosine arabinoside, which are used in the treatment of certain childhood leukemias. This resistance can impact the effectiveness of chemotherapy treatments, making it a significant area of study in cancer research.

Recombinant Cytidine Deaminase

Human recombinant cytidine deaminase is produced using recombinant DNA technology, which involves inserting the CDA gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the enzyme for research and therapeutic purposes. Recombinant cytidine deaminase is used in various biochemical assays and studies to understand its function and role in nucleotide metabolism.

Applications in Research

Cytidine deaminase is widely used in research to study nucleotide metabolism and the pyrimidine salvage pathway. It is also used in the development of gene-editing technologies, such as base editors, which utilize cytidine deaminases to introduce specific mutations in DNA. These technologies have significant potential for therapeutic applications, including the treatment of genetic disorders and cancers.

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CDA Human

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