HEK293 Cells.
CD99 antigen-like protein 2, MIC2-like protein 1, Cd99l2, Mic2l1, CD99 antigen-like protein 2 isoform 1, Xap89, Mic2l1.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
CD99L2 Mouse Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 378 amino acids (26-161a.a) and having a molecular mass of 41.8kDa. CD99L2 is fused to a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
CD99 antigen-like protein 2, MIC2-like protein 1, Cd99l2, Mic2l1, CD99 antigen-like protein 2 isoform 1, Xap89, Mic2l1.
HEK293 Cells.
DGSDTDGFNL EDALKETSSV KQRWDHFSTT TRRPVTTRAP ANPAERWDHV ATTTTRRPGT TRAPSNPMEL DGFDLEDALD DRNDLDGPKK PSAGEAGGWS DKDLEDIVEG GGYKPDKNKG GGGYGSNDDP GSGISTETGLEPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH.
The mouse CD99L2 cDNA encodes a 214 amino acid precursor with a 23 amino acid predicted signal sequence, a 116 amino acid extracellular domain (ECD), a 21 amino acid transmembrane segment, and a 54 amino acid cytoplasmic region . The protein has a molecular weight of approximately 45 kDa .
CD99L2 functions as an adhesion molecule and plays a crucial role in leukocyte extravasation, particularly during the diapedesis step . It acts independently of PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) and cooperates with CD99 to help neutrophils overcome the endothelial basement membrane . This protein is not involved in the docking of leukocytes to the vessel wall or in lymphocyte diapedesis .
Recombinant mouse CD99L2 is produced using various expression systems, such as HEK293 cells or mouse myeloma cell lines . The recombinant protein is often tagged with a His tag or Fc chimera to facilitate purification and detection . It is typically lyophilized from a sterile PBS solution and can be reconstituted for use in various research applications .
Recombinant mouse CD99L2 is used in various research areas, including immunology, cell biology, and molecular biology. It is particularly useful in studying leukocyte-endothelial cell interactions and the mechanisms of leukocyte extravasation . The protein’s ability to bind biotinylated recombinant mouse CD99L2 in functional ELISA assays makes it a valuable tool for biochemical and cellular studies .
Lyophilized recombinant mouse CD99L2 is stable for up to 12 months when stored at -20 to -80°C . Once reconstituted, the protein solution can be stored at 4-8°C for 2-7 days or at -20°C for up to 3 months . It is essential to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity .