CD99 Human
Sf9, Baculovirus cells.
CD99 antigen, T-cell surface glycoprotein E2, E2 antigen, Protein MIC2, 12E7, CD99, MIC2, MIC2X, MIC2Y.
Greater than 95.0% as determined by SDS-PAGE.
Description
CD99 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 342 amino acids (23-122a.a.) and having a molecular mass of 37.3kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
CD99 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD99 antigen, T-cell surface glycoprotein E2, E2 antigen, Protein MIC2, 12E7, CD99, MIC2, MIC2X, MIC2Y.
Sf9, Baculovirus cells.
ADPDGGFDLS DALPDNENKK PTAIPKKPSA GDDFDLGDAV VDGENDDPRP PNPPKPMPNP NPNHPSSSGS FSDADLADGV SGGEGKGGSD GGGSHRKEGE EADLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.
Product Science Overview
Native human CD99 is approximately 32 kDa in size and exists as a type I transmembrane glycoprotein . The recombinant form of CD99 is often produced in a mouse myeloma cell line (NS0-derived) and is typically purified to a high degree of purity, often greater than 90% as determined by SDS-PAGE under reducing conditions . The recombinant protein is usually lyophilized from a filtered solution in PBS and can be reconstituted in sterile PBS for use in various applications .
CD99 is involved in several essential cellular functions, including:
- Cell Adhesion and Migration: CD99 plays a role in the adhesion and migration of cells, which is critical for immune responses and tissue repair .
- Cell Death and Differentiation: It is involved in the regulation of cell death and differentiation, which is important for maintaining cellular homeostasis .
- Intracellular Protein Trafficking: CD99 is implicated in the trafficking of proteins within cells, affecting processes such as endocytosis and exocytosis .
CD99 is notably expressed in Ewing sarcoma (EWS) cells, a type of pediatric bone tumor. Targeting CD99 in EWS cells has been shown to inhibit tumor growth by inducing cell death and promoting phagocytosis by macrophages . This makes CD99 a potential therapeutic target for treating EWS and possibly other cancers.
Recombinant human CD99 is widely used in research to study its functions and potential therapeutic applications. It is often used in functional assays, such as ELISA, to measure its binding activity and interactions with other molecules . Additionally, CD99’s role in immune cell regulation and tumor biology makes it a valuable target for developing new cancer therapies .
