CD96 Human
Sf9, Baculovirus cells.
CD96 Molecule, T Cell-Activated Increased Late Expression Protein, Cell Surface Antigen CD96, CD96 Antigen, T Cell Activation, Increased Late Expression, T-Cell Surface Protein Tactile, TACTILE.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD96 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 490 amino acids (22-503a.a.) and having a molecular mass of 54.6kDa.
CD96 is expressed with an 8 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD96 Molecule, T Cell-Activated Increased Late Expression Protein, Cell Surface Antigen CD96, CD96 Antigen, T Cell Activation, Increased Late Expression, T-Cell Surface Protein Tactile, TACTILE.
Sf9, Baculovirus cells.
VWEKTVNTEE NVYATLGSDV NLTCQTQTVG FFVQMQWSKV TNKIDLIAVY HPQYGFYCAY GRPCESLVTF TETPENGSKW TLHLRNMSCS VSGRYECMLV LYPEGIQTKI YNLLIQTHVT ADEWNSNHTI EIEINQTLEI PCFQNSSSKI SSEFTYAWSV EDNGTQETLI SQNHLISNST LLKDRVKLGT DYRLHLSPVQ IFDDGRKFSC HIRVGPNKIL RSSTTVKVFA KPEIPVIVEN NSTDVLVERR FTCLLKNVFP KANITWFIDG SFLHDEKEGI YITNEERKGK DGFLELKSVL TRVHSNKPAQ SDNLTIWCMA LSPVPGNKVW NISSEKITFL LGSEISSTDP PLSVTESTLD TQPSPASSVS PARYPATSSV TLVDVSALRP NTTPQPSNSS MTTRGFNYPW TSSGTDTKKS VSRIPSETYS SSPSGAGSTL HDNVFTSTAR AFSEVPTTAN GSTKTNHVHI TGIVVNKPKD GMLEHHHHHH.
Product Science Overview
The human CD96 protein consists of 493 amino acids and has a predicted molecular mass of approximately 55 kDa . However, due to extensive glycosylation, the apparent molecular mass of recombinant human CD96 (rhCD96) is around 120-130 kDa when analyzed by SDS-PAGE under reducing conditions . The extracellular domain of CD96 is responsible for its interaction with other molecules, such as CD155 (also known as PVR), which is important for its function in the immune response .
CD96 expression peaks 6-9 days after the activation of T cells, NK cells, and a subpopulation of B cells . This delayed expression suggests that CD96 may play a role in the later stages of immune responses, potentially contributing to the regulation of immune cell interactions and signaling.
CD96 is involved in several key functions within the immune system:
- Cell Adhesion: CD96 mediates cell-cell adhesion by binding to its ligand, CD155. This interaction is important for the formation of immune synapses and the regulation of immune cell interactions .
- Immune Signaling: CD96 can initiate signaling cascades that influence the behavior of immune cells. This includes the activation and proliferation of T cells and NK cells, which are essential for effective immune responses .
- Immune Regulation: CD96 may also play a role in the regulation of immune responses by modulating the activity of T cells and NK cells. This can help maintain immune homeostasis and prevent excessive immune activation .
Recombinant human CD96 (rhCD96) is produced using DNA sequences encoding the extracellular domain of human CD96. This recombinant protein is typically expressed in HEK293 cells and purified to high purity levels, often exceeding 90% as determined by SDS-PAGE and 95% as determined by SEC-HPLC . The recombinant protein is commonly used in research to study the function and interactions of CD96 in the immune system.
Recombinant human CD96 has several applications in scientific research:
- Functional Studies: rhCD96 can be used in functional assays to study its binding interactions with ligands such as CD155. This helps researchers understand the role of CD96 in immune cell adhesion and signaling .
- Immunophenotyping: CD96 is used as a marker in immunophenotyping to identify and characterize different immune cell populations. This is important for studying immune responses and identifying specific cell types involved in various diseases .
- Therapeutic Research: CD96 is being investigated as a potential target for immunotherapy. By modulating CD96 interactions, researchers aim to develop new treatments for cancer and other immune-related disorders .
