CD83 Human
CD83 Human Recombinant
Cat. No.
BT4316
Source
E.coli.
Synonyms
BL11, HB15, hCD83, B-cell activation protein, Cell surface protein HB15.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CD83 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids (20-144a.a) and having a molecular mass of 16kDa. CD83 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD83 is a member of the immunoglobulin superfamily and functions as a single-pass type I membrane protein. It plays a crucial role in regulating antigen presentation. The soluble form of CD83 can bind to dendritic cells, inhibiting their maturation process. CD83 is expressed in three transcript variants, each encoding a distinct isoform.
Description
Recombinant human CD83, expressed in E. coli, is a non-glycosylated polypeptide chain with a molecular weight of 16 kDa. It consists of 148 amino acids, encompassing residues 20 to 144, and includes a 23 amino acid His-tag fused at the N-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for clarity.
Formulation
The CD83 protein solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, and 30% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the CD83 protein is greater than 90%, as assessed by SDS-PAGE analysis.
Synonyms
BL11, HB15, hCD83, B-cell activation protein, Cell surface protein HB15.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSTPEVKVA CSEDVDLPCT APWDPQVPYT VSWVKLLEGG EERMETPQED HLRGQHYHQK GQNGSFDAPN ERPYSLKIRN TTSCNSGTYR CTLQDPDGQR NLSGKVILRV TGCPAQRKEE TFKKYRAE.
Product Science Overview
Structure and Forms
CD83 exists in two primary forms:
- Membrane-bound CD83 (mCD83): This form is predominantly expressed on the surface of mature dendritic cells (DCs) and other activated immune cells. It consists of an extracellular V-type immunoglobulin-like domain, a transmembrane domain, and a cytoplasmic signaling tail . Membrane-bound CD83 is known to form trimers.
- Soluble CD83 (sCD83): This form is found in the serum and consists of the immunoglobulin-like domain alone. Soluble CD83 can assemble into dodecameric complexes .
Function
CD83 is involved in several critical functions within the immune system:
- Regulation of Dendritic Cells: Membrane-bound CD83 is highly and stably expressed by mature dendritic cells, where it regulates their maturation, activation, and homeostasis .
- Immune Modulation: Soluble CD83 has been reported to have an immunosuppressive function, which is particularly elevated in patients with autoimmune diseases and certain hematological malignancies .
- Stabilization of Immune Molecules: The transmembrane domain of membrane-bound CD83 stabilizes major histocompatibility complex (MHC) class II, costimulatory molecules, and CD28 in the membrane by antagonizing MARCH-family E3 ubiquitin ligases .
Therapeutic Potential
CD83 has emerged as a promising target for therapeutic interventions due to its role in immune regulation:
- Autoimmune Diseases: The immunosuppressive properties of soluble CD83 make it a potential therapeutic agent for treating autoimmune diseases .
- Graft-Versus-Host Disease (GVHD): Anti-CD83 specific antibodies have shown efficacy in pre-clinical models of GVHD by depleting CD83+ cells without significantly affecting viral or tumor-specific memory T-cell responses .
- Transplantation: CD83-based therapeutics could be used to prevent donor organ rejection by selectively suppressing undesirable immune responses while preserving beneficial responses against infections and cancer .
Recombinant CD83
Recombinant human CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid extracellular region, a 22 amino acid transmembrane segment, and a 39 amino acid cytoplasmic domain . This recombinant form is used in research and therapeutic applications to study and harness the immune-modulating properties of CD83.
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