CD80 Human
Sf9, Insect cells.
CD80 Molecule, CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen), B-Lymphocyte Activation Antigen B7, CTLA-4 Counter-Receptor B7.1, Activation B7-1 Antigen, CD28LG1, CD28LG, LAB7, BB1, B7, Costimulatory Molecule Variant IgV-CD80, T-Lymphocyte Activation Antigen CD80, Costimulatory Factor CD80, CD80 Antigen, B7-1, B7.1.
Greater than 95.0% as determined by SDS-PAGE.
Description
CD80 Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 216 amino acids (35-242a.a.) and having a molecular mass of 24.9kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
CD80 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD80 Molecule, CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen), B-Lymphocyte Activation Antigen B7, CTLA-4 Counter-Receptor B7.1, Activation B7-1 Antigen, CD28LG1, CD28LG, LAB7, BB1, B7, Costimulatory Molecule Variant IgV-CD80, T-Lymphocyte Activation Antigen CD80, Costimulatory Factor CD80, CD80 Antigen, B7-1, B7.1.
Sf9, Insect cells.
VIHVTKEVKE VATLSCGHNV SVEELAQTRI YWQKEKKMVL TMMSGDMNIW PEYKNRTIFD ITNNLSIVIL ALRPSDEGTY ECVVLKYEKD AFKREHLAEV TLSVKADFPT PSISDFEIPT SNIRRIICST SGGFPEPHLS WLENGEELNA INTTVSQDPE TELYAVSSKL DFNMTTNHSF MCLIKYGHLR VNQTFNWNTT KQEHFPDNLE HHHHHH.
Product Science Overview
CD80, also known as B7-1, is a type I membrane protein that belongs to the immunoglobulin superfamily. It plays a crucial role in the regulation of T-cell activation and immune responses. CD80 is primarily expressed on antigen-presenting cells (APCs) such as dendritic cells, activated B-cells, and macrophages .
CD80 is a transmembrane glycoprotein with an extracellular domain that includes an immunoglobulin constant-like domain and a variable-like domain required for receptor binding . It is closely related to CD86 (B7-2), and both molecules often work in tandem to regulate immune responses .
The primary function of CD80 is to provide co-stimulatory signals necessary for T-cell activation and survival. It achieves this by binding to receptors on T-cells, specifically CD28 and CTLA-4. The interaction between CD80 and CD28 provides a positive co-stimulatory signal that promotes T-cell activation and proliferation. Conversely, the interaction between CD80 and CTLA-4 delivers an inhibitory signal that downregulates T-cell responses .
Recombinant CD80 is produced using various expression systems, including human embryonic kidney (HEK293) cells. The recombinant protein is typically tagged with a polyhistidine (His) tag to facilitate purification and detection . The recombinant form of CD80 retains its ability to bind to its receptors, making it a valuable tool for research and therapeutic applications.
Recombinant CD80 is widely used in immunological research to study T-cell activation and co-stimulation. It is also employed in the development of immunotherapies for cancer and autoimmune diseases. By modulating the interactions between CD80 and its receptors, researchers can influence immune responses to achieve therapeutic benefits .
