CD74 Human, Sf9
CD74 Human Recombinant, Sf9
Cat. No.
BT30721
Source
Sf9, Baculovirus cells.
Synonyms
DHLAG, HLADG, Ia-GAMMA, li, HLA-DR antigens-associated
invariant chain, p33, Ia antigen-associated invariant chain, HLA class II
histocompatibility antigen gamma chain, CD74, Gamma Chain Of Class II Antigens,
MHC HLA-DR Gamma Chain.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CD74 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 166 amino acids (73-232a.a.) and
having a molecular mass of 19.05kDa (Molecular size on SDS-PAGE will appear at
approximately 18-28kDa). CD74 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
CD74, also known as HLA class II histocompatibility antigen gamma chain isoform b, is a transmembrane protein primarily found on antigen-presenting cells. It plays a crucial role in the immune system by chaperoning MHC class II molecules from the endoplasmic reticulum to the cell surface, where they present antigens to T cells. CD74 is expressed by both T and B lymphocytes and can be upregulated in various cancers and inflammatory conditions. Besides its role in antigen presentation, CD74 also serves as a receptor for the cytokine MIF (macrophage migration inhibitory factor). Dysregulation of CD74 is implicated in diseases such as thymic epithelial neoplasm and gastroduodenitis. Key pathways associated with CD74 include the immune system and immune response, particularly antigen presentation by MHC class II.
Description
This product consists of the CD74 protein produced in Sf9 insect cells using a baculovirus expression system. The protein is a single, glycosylated polypeptide chain containing 166 amino acids (residues 73-232) with a molecular weight of 19.05 kDa. However, it appears as a band at approximately 18-28 kDa on SDS-PAGE due to glycosylation. This CD74 protein is engineered with a 6-amino acid Histidine tag at the C-terminus to facilitate purification, which is carried out using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
This CD74 protein solution has a concentration of 0.5 mg/ml and is formulated in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. To ensure stability during long-term storage, consider adding a carrier protein like HSA or BSA at a concentration of 0.1%. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the CD74 protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
DHLAG, HLADG, Ia-GAMMA, li, HLA-DR antigens-associated
invariant chain, p33, Ia antigen-associated invariant chain, HLA class II
histocompatibility antigen gamma chain, CD74, Gamma Chain Of Class II Antigens,
MHC HLA-DR Gamma Chain.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
QQQGRLDKLT VTSQNLQLEN LRMKLPKPPK PVSKMRMATP LLMQALPMGA
LPQGPMQNAT KYGNMTEDHV MHLLQNADPL KVYPPLKGSF PENLRHLKNT METIDWKVFE SWMHHWLLFE
MSRHSLEQKP TDAPPKESLE LEDPSSGLGV TKQDLGPVPM HHHHHH.
Product Science Overview
Structure and Expression
The recombinant human CD74 protein produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 166 amino acids (73-232a.a.) and has a molecular mass of approximately 19.05 kDa. When analyzed using SDS-PAGE, the molecular size appears to be between 18-28 kDa . The protein is expressed with a 6 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .
Function
CD74 plays a crucial role in the immune system. It is involved in the formation and transport of MHC class II proteins, which are essential for the immune response. The protein acts as a chaperone, stabilizing the MHC class II molecules and preventing premature peptide binding. It also facilitates the transport of these molecules to the cell surface, where they present antigens to T cells .
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