CD69 Human

CD69 Human Recombinant
Cat. No.
BT3956
Source
Sf9, Baculovirus cells.
Synonyms
CD69 Molecule, CD69 Antigen (P60, Early T-Cell Activation Antigen), Early T-Cell Activation Antigen P60, Leukocyte Surface Antigen Leu-23, BL-AC/P26, GP32/28, CLEC2C, MLR-3, AIM, EA1, C-Type Lectin Domain Family 2, Member C, Activation Inducer Molecule (AIM/CD69), C-Type Lectin Domain Family 2 Member C, Early Lymphocyte Activation Antigen, Activation Inducer Molecule, CD69 Antigen, Early activation antigen CD69.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CD69 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 147 amino acids (62-199a.a.) and having a molecular mass of 17.0kDa. 
(Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
CD69 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD69 is a cell surface receptor that plays a role in the activation and regulation of immune cells, including T lymphocytes and natural killer cells. It is a member of the C-type lectin family and is expressed upon T cell activation, contributing to their proliferation. CD69 is also involved in signal transduction in natural killer cells and platelets. Dysregulation of CD69 has been implicated in allergic airway inflammation and other immune-related disorders.
Description
Recombinant human CD69, encompassing amino acids 62-199, has been expressed in Sf9 insect cells using baculovirus technology. This production method yields a glycosylated polypeptide chain with a molecular weight of approximately 17.0 kDa, as determined by mass spectrometry. The apparent molecular weight on SDS-PAGE analysis is expected to be between 18-28 kDa due to glycosylation. For purification purposes, a 6-amino acid Histidine tag has been incorporated at the C-terminus of the protein. Purification to greater than 95% purity has been achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The CD69 protein is provided as a solution at a concentration of 0.5 mg/mL in a buffer consisting of phosphate-buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the CD69 protein should be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. The addition of a carrier protein such as albumin (0.1% HSA or BSA) is advised for long-term storage to enhance protein stability. Repeated freezing and thawing of the protein solution should be avoided.
Purity
The purity of the CD69 protein is greater than 95.0%, as assessed by SDS-PAGE analysis.
Synonyms
CD69 Molecule, CD69 Antigen (P60, Early T-Cell Activation Antigen), Early T-Cell Activation Antigen P60, Leukocyte Surface Antigen Leu-23, BL-AC/P26, GP32/28, CLEC2C, MLR-3, AIM, EA1, C-Type Lectin Domain Family 2, Member C, Activation Inducer Molecule (AIM/CD69), C-Type Lectin Domain Family 2 Member C, Early Lymphocyte Activation Antigen, Activation Inducer Molecule, CD69 Antigen, Early activation antigen CD69.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPSVGQYNC PGQYTFSMPS DSHVSSCSED WVGYQRKCYF ISTVKRSWTS AQNACSEHGA TLAVIDSEKD MNFLKRYAGR EEHWVGLKKE PGHPWKWSNG KEFNNWFNVT GSDKCVFLKN TEVSSMECEK NLYWICNKPY KHHHHHH.

Product Science Overview

Structure and Expression

CD69 is a disulfide-linked homodimer with a predicted molecular mass of approximately 17 kDa. However, due to glycosylation, it migrates as 20-30 kDa under reducing conditions in SDS-PAGE . The protein is expressed on the surface of activated T-cells, B-cells, natural killer (NK) cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets .

Activation and Function

CD69 is rapidly induced upon activation by antigens, mitogens, or activators of protein kinase C (PKC). It is also induced by the interaction of interleukin-2 (IL-2) with the p75 IL-2 receptor on the surface of NK cells . CD69 functions as a signal-transmitting receptor in lymphocytes, NK cells, and platelets, and is involved in lymphocyte proliferation .

Recombinant Human CD69

Recombinant human CD69 is produced using various expression systems, including mouse myeloma cell lines (NS0-derived) and human 293 cells (HEK293). The recombinant protein is often tagged with a polyhistidine (His) tag for purification purposes . The purity of the recombinant protein is typically greater than 95%, as determined by SDS-PAGE .

Applications

Recombinant human CD69 is used in various research applications, including functional assays, binding studies, and as a standard in enzyme-linked immunosorbent assays (ELISAs). It is also used to study the role of CD69 in immune responses and its potential as a therapeutic target .

Storage and Stability

Recombinant human CD69 is typically lyophilized from a filtered solution in phosphate-buffered saline (PBS) and can be reconstituted in PBS for use. It is stable for up to 12 months when stored at -20°C to -70°C in its lyophilized state and for up to 3 months after reconstitution under sterile conditions .

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