CD6 Human
Sf9, Baculovirus cells.
T-cell differentiation antigen CD6, T12, TP120, CD6, CD antigen, T-cell differentiation antigen CD6 isform1
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD6 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 627 amino acids (18-402a.a.) and having a molecular mass of 68.3kDa.
CD6 is expressed with a 239 amino acid hIgG-His-Tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD6 is a glycoprotein found on the surface of T cells, playing a crucial role in their function. It is also present on thymocytes (immature T cells), a specific subset of B cells (B-1 cells), and certain brain cells. As a member of the SRCR superfamily, CD6 participates in cell signaling and adhesion. It interacts with CD166, contributing to T cell activation and the development of T cells in the thymus.
This recombinant CD6 protein, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. It consists of 627 amino acids (specifically, amino acids 18-402a), resulting in a molecular weight of 68.3 kDa. The protein includes a 239 amino acid hIgG-His-Tag at its C-terminus and undergoes purification through proprietary chromatographic methods.
A clear, colorless solution that has been sterilized by filtration.
The CD6 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution also contains 10% glycerol and Phosphate-Buffered Saline (PBS) with a pH of 7.4.
For short-term storage (up to 2-4 weeks), keep the vial refrigerated at 4°C. For extended storage, freeze the protein at -20°C. Adding a carrier protein (either 0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing of the protein should be avoided.
The purity of the CD6 protein is greater than 90% as determined by SDS-PAGE analysis.
The protein exhibits significant biological activity, as demonstrated by its ability to support the adhesion of Jurkat cells (a human acute T cell leukemia cell line). When Jurkat cells are added to plates coated with human CD6 at a concentration of 10 µg/ml, the adhesion is enhanced by more than 50%. This adhesion-promoting activity is a key characteristic of functional CD6 protein.
T-cell differentiation antigen CD6, T12, TP120, CD6, CD antigen, T-cell differentiation antigen CD6 isform1
Sf9, Baculovirus cells.
ADPHPSPAPP DQLNTSSAES ELWEPGERLP VRLTNGSSSC SGTVEVRLEA SWEPACGALW DSRAAEAVCR ALGCGGAEAA SQLAPPTPEL PPPPAAGNTS VAANATLAGA PALLCSGAEW RLCEVVEHAC RSDGRRARVT CAENRALRLV DGGGACAGRV EMLEHGEWGS VCDDTWDLED AHVVCRQLGC GWAVQALPGL HFTPGRGPIH RDQVNCSGAE AYLWDCPGLP GQHYCGHKED AGAVCSEHQS WRLTGGADRC EGQVEVHFRG VWNTVCDSEW YPSEAKVLCQ SLGCGTAVER PKGLPHSLSG RMYYSCNGEE LTLSNCSWRF NNSNLCSQSL AARVLCSASR SLHNLSTPEV PASVQTVTIE SSVTVKIENK ESRELMLLVE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH
Product Science Overview
CD6, also known as T-cell differentiation antigen CD6, is a type I transmembrane glycoprotein that plays a crucial role in the immune system. It is primarily expressed on the surface of T cells, a subset of B cells known as B1a cells, and natural killer (NK) cells. CD6 is also found on certain hematopoietic precursors and in specific regions of the brain .
The CD6 protein consists of three scavenger receptor cysteine-rich (SRCR) domains. These domains are essential for its function and interactions with other molecules. The membrane-proximal domain (D3) of CD6 binds to the amino-terminal domain (D1) of activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 . This interaction is critical for the stabilization of cell-to-cell contacts and modulation of activation and differentiation events in the immune response .
CD6 is involved in the continuation of T cell activation and plays a role in the immune response. It acts as a co-stimulatory molecule, enhancing the activation and proliferation of T cells. CD6 also assists in the stabilization of the immunological synapse, which is the interface between T cells and antigen-presenting cells .
Recombinant human CD6 is a form of the CD6 protein that is produced using recombinant DNA technology. This involves inserting the gene encoding CD6 into a host cell, such as HEK293 cells, which then express the protein. The recombinant protein is typically purified and used for various research and therapeutic purposes .
Recombinant human CD6 has several applications in biomedical research and therapy. It is used to study the role of CD6 in immune responses and to develop potential therapeutic strategies for autoimmune diseases and cancer. For example, soluble forms of CD6 (sCD6) have been explored as decoy receptors to block CD6-mediated interactions, which can modulate antitumor lymphocyte effector function and tumorigenesis .
