Introduction
CD59 is a cell surface glycoprotein that plays a crucial role in regulating complement-mediated cell lysis and participates in lymphocyte signal transduction. As an effective inhibitor of the complement membrane attack complex, CD59 binds to complement components C8 and/or C9 during the assembly of this complex. This binding prevents the integration of multiple C9 molecules into the complex, which is a necessary step for the formation of pores that cause cell lysis. Additionally, CD59 contributes to signal transduction pathways involved in T cell activation. Mutations in the CD59 gene can lead to CD59 deficiency, a disorder characterized by hemolytic anemia, thrombosis, and an increased risk of cerebral infarction.
Description
Recombinant human CD59, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 100 amino acids (residues 26-102). It has a molecular weight of 11.3 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The CD59 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability, adding a carrier protein (0.1% HSA or BSA) is advised. Repeated freezing and thawing cycles should be avoided.
Purity
The purity of the protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
CD59 Molecule Complement Regulatory Protein, CD59 Antigen P18-20, CD59 Glycoprotein, Surface Anitgen Recognized By Monoclonal Antibody 16.3A5, Membrane Attack Complex (MAC) Inhibition Factor, Lymphocytic Antigen CD59/MEM43, Human Leukocyte Antigen MIC11, Membrane Attack Complex Inhibition Factor, 20 KDa Homologous Restriction Factor, Membrane Inhibitor Of Reactive Lysis, MAC-Inhibitory Protein, T Cell-Activating Protein, Ly-6-Like Protein, Protectin, MIC11, MSK21, MEM43, MIN1/2/3, MIRL, MAC-IP, MACIF, HRF-20, G344, EJ16/30/32, 1F5.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSLQCYNCP NPTADCKTAV NCSSDFDACL ITKAGLQVYN KCWKFEHCNF NDVTTRLREN ELTYYCCKKD LCNFNEQLEN.
